一种作为小鼠胚胎干细胞基因靶向选择标记的pgk::hprt融合基因：T细胞受体δ链编码基因的破坏
A pgk::hprt fusion as a selectable marker for targeting of genes in mouse embryonic stem cells: disruption of the T-cell receptor delta-chain-encoding gene.
作者信息
van der Lugt N, Maandag E R, te Riele H, Laird P W, Berns A
机构信息
Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam.
出版信息
Gene. 1991 Sep 15;105(2):263-7. doi: 10.1016/0378-1119(91)90161-4.
We have constructed a hypoxanthine phosphoribosyl transferase-selectable marker (hprt) under the control of the phosphoglycerate kinase (pgk) promoter. This construct permits cell growth in hypoxanthine/aminopterin/thymidine media and confers 6-thioguanine sensitivity upon mouse Hprt- embryonic stem cells, allowing either positive or negative selection in gene-targeting experiments. We have successfully targeted the gene encoding the T-cell receptor delta-chain using the pgk::hprt fusion for counterselection.
我们构建了一个在磷酸甘油酸激酶（pgk）启动子控制下的次黄嘌呤磷酸核糖基转移酶选择标记（hprt）。该构建体允许细胞在次黄嘌呤/氨基蝶呤/胸腺嘧啶核苷培养基中生长，并赋予小鼠Hprt-胚胎干细胞对6-硫鸟嘌呤的敏感性，从而在基因靶向实验中实现正向或负向选择。我们已经成功地使用pgk::hprt融合体进行反选择，对编码T细胞受体δ链的基因进行了靶向。
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