即时检验中使用糖原磷酸化酶BB检测诊断急性冠脉综合征。与酶联免疫吸附测定法的比较。

Use of glycogen phosphorylase BB measurement with POCT in the diagnosis of acute coronary syndromes. A comparison with the ELISA method.

作者信息

Stejskal David, Lacnak Borek, Jedelsky Libor, Stepanova Leona, Proskova Jitka, Solichova Pavlina, Kadalova Lenka, Janosova Marie, Seitlova Petra, Karpisek Michal, Sprongl Ludek

机构信息

Department of Laboratory Medicine, Sternberk Hospital, Czech Republic.

出版信息

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2007 Dec;151(2):247-9. doi: 10.5507/bp.2007.041.

DOI:10.5507/bp.2007.041

PMID:18345258

Abstract

BACKGROUND

Glycogen Phosphorylase BB (GPBB) is considered an early and specific marker of myocardial necrosis and ischemia. A POCT kit GPBB for diagnostic use has recently been approved.

AIM

an evaluation of the correspondence of qualitative POCT GBPP measurements with ELISA test results.

MATERIAL AND METHODOLOGY

20 individuals with non-ST elevation myocardial infarction (non-STEMI) and 20 probands without acute coronary syndrome (ACS) were tested. GPBB (POCT, ELISA) in venous plasma (lithium-heparin) was assayed in all probands.

RESULTS

individuals with non-STEMI had significantly higher GPBB ELISA values (32.3 vs. 6.1 microg/l; p < 0.01). GPBB sensitivity and specificity for non-STEMI presence 6 hours after chest pain generation were 100 %. No proband was classified in a different subgroup with POCT of GPBB (positive/negative). GPBB POCT correlate with a non- STEMI diagnosis (chi(2) 36.1; p <0.01).

CONCLUSION

GPBB POCT measurement is comparable with ELISA test results. GPBB analysis could expand the diagnostic palette in the first hours after the onset of acute coronary syndrome.

摘要

背景

糖原磷酸化酶BB（GPBB）被认为是心肌坏死和缺血的早期特异性标志物。一种用于诊断的即时检验（POCT）试剂盒GPBB最近已获批准。

目的

评估定性POCT法检测GBPP的结果与酶联免疫吸附测定（ELISA）试验结果的一致性。

材料与方法

对20例非ST段抬高型心肌梗死（非STEMI）患者和20例无急性冠状动脉综合征（ACS）的对照者进行检测。对所有受试者的静脉血浆（锂肝素抗凝）进行GPBB检测（POCT法、ELISA法）。

结果

非STEMI患者的GPBB ELISA值显著更高（32.3对6.1微克/升；p<0.01）。胸痛发作6小时后，GPBB对非STEMI的敏感性和特异性均为100%。在GPBB的POCT检测中（阳性/阴性），没有受试者被归为不同亚组。GPBB的POCT检测结果与非STEMI诊断相关（χ²=36.1；p<0.01）。