Desprès P, Dietrich J, Girard M, Bouloy M
Unité de Virologie Moléculaire, CNRS URA 545, Institut Pasteur, Paris, France.
J Gen Virol. 1991 Nov;72 ( Pt 11):2811-6. doi: 10.1099/0022-1317-72-11-2811.
Recently, we showed that yellow fever virus (YFV) E and NS1 proteins in Spodoptera frugiperda cells infected with a recombinant baculovirus are similar, if not identical to those produced during YFV infection. To study the role of E and NS1 in the induction of protective immunity against fatal YFV challenge, these viral antigens were expressed either alone or in tandem via recombinant baculoviruses Ac-E. NS1, Ac-E1 and Ac-NS1. Swiss mice were immunized with lysates of insect cells infected with the recombinant baculoviruses. Solid protection against lethal YFV encephalitis was achieved after immunization with cell lysates containing the E protein with or without the NS1 protein. Mice inoculated with recombinant protein NS1 alone were not significantly protected but showed an increased survival time. Recombinant E protein expressed alone or in tandem with NS1 elicited a low but significant level of neutralizing antibodies. Although protein NS1 synthesized by recombinant baculovirus expressing E plus NS1 was more immunogenic than that expressed alone, neither strategy induced NS1-specific antibodies with complement-mediated cytolytic activity.
最近，我们发现，感染重组杆状病毒的草地贪夜蛾细胞中产生的黄热病毒（YFV）E蛋白和NS1蛋白，即便不完全相同，也与YFV感染期间产生的蛋白相似。为了研究E蛋白和NS1蛋白在诱导针对致命YFV攻击的保护性免疫中的作用，这些病毒抗原通过重组杆状病毒Ac-E. NS1、Ac-E1和Ac-NS1单独或串联表达。用感染了重组杆状病毒的昆虫细胞裂解物对瑞士小鼠进行免疫。用含有E蛋白（无论有无NS1蛋白）的细胞裂解物免疫后，可实现对致命YFV脑炎的有效保护。单独接种重组蛋白NS1的小鼠没有得到显著保护，但存活时间有所延长。单独表达或与NS1串联表达的重组E蛋白引发了低水平但显著的中和抗体。尽管表达E加NS1的重组杆状病毒合成的蛋白NS1比单独表达的更具免疫原性，但两种策略均未诱导出具有补体介导的细胞溶解活性的NS1特异性抗体。
