McCown J, Cochran M, Putnak R, Feighny R, Burrous J, Henchal E, Hoke C
Walter Reed Army Institute of Research, Washington, DC.
Am J Trop Med Hyg. 1990 May;42(5):491-9. doi: 10.4269/ajtmh.1990.42.491.
Genes coding for the E and NS1 glycoproteins of Japanese encephalitis virus (JEV) were cloned into baculovirus expression vectors. The recombinant baculoviruses obtained were used to infect Spodoptera frugiperda cells. The infected cells were used to immunize C57/B mice, which were then challenged with live JEV. Survival was increased from about 30% in unimmunized mice to 70% in E and polyprotein recipients (P less than 0.005), but was not increased in NS1 recipients despite the development of antibody against NS1 by these mice. Virus neutralizing antibody was demonstrated in 18/20 E glycoprotein recipients and 15/20 polyprotein recipients. The baculovirus expressed E glycoprotein stimulated antibody which was protective and neutralizing in this system.
将编码日本脑炎病毒（JEV）E糖蛋白和NS1糖蛋白的基因克隆到杆状病毒表达载体中。获得的重组杆状病毒用于感染草地贪夜蛾细胞。用感染的细胞免疫C57/B小鼠，然后用活的JEV攻击这些小鼠。未免疫小鼠的存活率约为30%，而接受E糖蛋白和多蛋白免疫的小鼠存活率提高到70%（P<0.005），尽管接受NS1免疫的小鼠产生了抗NS1抗体，但其存活率并未提高。在20只接受E糖蛋白免疫的小鼠中有18只、20只接受多蛋白免疫的小鼠中有15只检测到病毒中和抗体。杆状病毒表达的E糖蛋白刺激产生的抗体在该系统中具有保护性和中和性。
