Obika Mikako, Shinji Toshiyuki, Fujioka Shin-Ichi, Terada Ryo, Ryuko Hiromasa, Lwin Aye Aye, Shiraha Hidenori, Koide Norio
Department of Laboratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Intervirology. 2008;51(1):59-68. doi: 10.1159/000121363. Epub 2008 Mar 18.
To prospectively study whether occult hepatitis B virus (HBV) infection can promote the development of hepatocellular carcinoma (HCC) in patients with hepatitis C virus (HCV)-related chronic liver disease. In addition, to evaluate the difference among HBV DNA-negative patients and patients with high and low HBV copy numbers.
A total of 167 patients with HCV-related chronic liver disease without HBV surface antigen (HBsAg) were studied. HBV DNA in liver tissue was determined using polymerase chain reaction (PCR).
HBV DNA was detected in 9 of 167 patients (5.4%) by single PCR and in 25 patients (15.0%) by nested PCR. HCC developed in 12 of 167 patients (7.2%). Ten of 142 HBV DNA-negative patients (7.0%) and 2 of 9 patients with a high HBV copy number (22.2%) developed HCC, whereas none of 16 patients with a low HBV copy number developed HCC. The incidence rate of HCC in patients with a high HBV copy number was significantly higher than in HBV DNA-negative patients and patients with low HBV copy number.
A high amount of HBV DNA in liver tissue of HBsAg-negative patients with HCV-related liver disease might be associated with HCC development.
前瞻性研究隐匿性乙型肝炎病毒（HBV）感染是否会促进丙型肝炎病毒（HCV）相关慢性肝病患者肝细胞癌（HCC）的发生。此外，评估HBV DNA阴性患者与高、低HBV拷贝数患者之间的差异。
共研究了167例无HBV表面抗原（HBsAg）的HCV相关慢性肝病患者。采用聚合酶链反应（PCR）检测肝组织中的HBV DNA。
167例患者中，单重PCR检测到9例（5.4%）HBV DNA，巢式PCR检测到25例（15.0%）。167例患者中有12例（7.2%）发生了HCC。142例HBV DNA阴性患者中有10例（7.0%）、9例高HBV拷贝数患者中有2例（22.2%）发生了HCC，而16例低HBV拷贝数患者均未发生HCC。高HBV拷贝数患者的HCC发病率显著高于HBV DNA阴性患者和低HBV拷贝数患者。
HBsAg阴性的HCV相关肝病患者肝组织中高含量的HBV DNA可能与HCC的发生有关。
