丙型肝炎病毒相关慢性肝病患者肝组织中的乙型肝炎病毒DNA与肝癌发生风险：一项前瞻性研究

Hepatitis B virus DNA in liver tissue and risk for hepatocarcinogenesis in patients with hepatitis C virus-related chronic liver disease. A prospective study.

作者信息

Obika Mikako, Shinji Toshiyuki, Fujioka Shin-Ichi, Terada Ryo, Ryuko Hiromasa, Lwin Aye Aye, Shiraha Hidenori, Koide Norio

机构信息

Department of Laboratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

出版信息

Intervirology. 2008;51(1):59-68. doi: 10.1159/000121363. Epub 2008 Mar 18.

DOI:10.1159/000121363

PMID:18349544

Abstract

AIMS

To prospectively study whether occult hepatitis B virus (HBV) infection can promote the development of hepatocellular carcinoma (HCC) in patients with hepatitis C virus (HCV)-related chronic liver disease. In addition, to evaluate the difference among HBV DNA-negative patients and patients with high and low HBV copy numbers.

METHODS

A total of 167 patients with HCV-related chronic liver disease without HBV surface antigen (HBsAg) were studied. HBV DNA in liver tissue was determined using polymerase chain reaction (PCR).

RESULTS

HBV DNA was detected in 9 of 167 patients (5.4%) by single PCR and in 25 patients (15.0%) by nested PCR. HCC developed in 12 of 167 patients (7.2%). Ten of 142 HBV DNA-negative patients (7.0%) and 2 of 9 patients with a high HBV copy number (22.2%) developed HCC, whereas none of 16 patients with a low HBV copy number developed HCC. The incidence rate of HCC in patients with a high HBV copy number was significantly higher than in HBV DNA-negative patients and patients with low HBV copy number.

CONCLUSION

A high amount of HBV DNA in liver tissue of HBsAg-negative patients with HCV-related liver disease might be associated with HCC development.

摘要

目的

前瞻性研究隐匿性乙型肝炎病毒（HBV）感染是否会促进丙型肝炎病毒（HCV）相关慢性肝病患者肝细胞癌（HCC）的发生。此外，评估HBV DNA阴性患者与高、低HBV拷贝数患者之间的差异。

方法

共研究了167例无HBV表面抗原（HBsAg）的HCV相关慢性肝病患者。采用聚合酶链反应（PCR）检测肝组织中的HBV DNA。

结果

167例患者中，单重PCR检测到9例（5.4%）HBV DNA，巢式PCR检测到25例（15.0%）。167例患者中有12例（7.2%）发生了HCC。142例HBV DNA阴性患者中有10例（7.0%）、9例高HBV拷贝数患者中有2例（22.2%）发生了HCC，而16例低HBV拷贝数患者均未发生HCC。高HBV拷贝数患者的HCC发病率显著高于HBV DNA阴性患者和低HBV拷贝数患者。