James C Andrew, Xin Gang, Doty Sharon L, Strand Stuart E
Department of Civil and Environmental Engineering, and College of Forest Resources, University of Washington, Seattle, Washington 98195, USA.
Environ Sci Technol. 2008 Jan 1;42(1):289-93. doi: 10.1021/es071197z.
Cytochrome P450 2E1 (CYP2E1) is a key enzyme in the mammalian metabolism of several low molecular weight volatile organic compounds (VOCs), such as trichloroethylene (TCE), vinyl chloride (VC), carbon tetrachloride (CT), benzene, chloroform, and bromodichloromethane (BDCM), which are all common environmental pollutants that pose risks to human health. We have developed a transgenic tobacco (Nicotiana tabacum cv. Xanthii) that expresses CYP2E1 with increased activity toward TCE and ethylene dibromide. In experiments with tobacco plant cuttings exposed to VOCs in small hydroponic vessels, the transgenic tobacco had greatly increased rates of removal of TCE, VC, CT, benzene, toluene, chloroform, and BDCM, compared to wild-type or vector control tobacco, but not of perchloroethylene or 1,1,1-trichloroethane.
细胞色素P450 2E1（CYP2E1）是哺乳动物代谢几种低分子量挥发性有机化合物（VOCs）的关键酶，这些化合物包括三氯乙烯（TCE）、氯乙烯（VC）、四氯化碳（CT）、苯、氯仿和溴二氯甲烷（BDCM），它们都是对人类健康构成风险的常见环境污染物。我们培育了一种转基因烟草（烟草品种Xanthii），它表达的CYP2E1对TCE和二溴乙烷的活性增强。在用小水培容器中暴露于VOCs的烟草插条进行的实验中，与野生型或载体对照烟草相比，转基因烟草对TCE、VC、CT、苯、甲苯、氯仿和BDCM的去除率大大提高，但对全氯乙烯或1,1,1 - 三氯乙烷的去除率没有提高。
