Tschentscher Frank, Frey Ulrich H, Bajanowski Thomas
Institute of Legal Medicine, University of Duisburg-Essen, Essen, Germany.
Int J Legal Med. 2008 Jul;122(4):333-5. doi: 10.1007/s00414-008-0228-4. Epub 2008 Mar 20.
We developed an assay, which allows the sex determination of human DNA samples by pyrosequencing of short PCR products. A 48/45-bp stretch including primers of the amelogenin gene with a 3-bp insertion on the Y chromosome was chosen for analysis. In an initial study, we correctly typed 50 male and 50 female DNA samples from unrelated donors. First experiments with forensic samples, which failed in conventional analyses, indicate that this approach might be an advantage when dealing with degraded DNA.
我们开发了一种检测方法，可通过对短PCR产物进行焦磷酸测序来确定人类DNA样本的性别。选择了一段48/45碱基对的片段，包括牙釉蛋白基因的引物，该引物在Y染色体上有一个3碱基对的插入，用于分析。在一项初步研究中，我们对来自无关供体的50份男性和50份女性DNA样本进行了正确分型。对传统分析失败的法医样本进行的首次实验表明，在处理降解DNA时，这种方法可能具有优势。
