Silverman Jerald, Bays David W, Cooper Sheldon F, Baker Stephen P
Department of Animal Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
J Am Assoc Lab Anim Sci. 2008 Mar;47(2):57-62.
This study compares resuable and disposable individually ventilated mouse cages in terms of the formation of intracage CO2 and NH3. Crl:CD-1(ICR) female mice were placed in either disposable or reusable ventilated cages in a positive pressure animal rack. Intracage CO2 and NH3 were measured once daily for 9 d; temperature and relative humidity were monitored for the first 7 d. Results indicated higher CO2 levels in the rear of the disposable cages and in the front of the reusable cages. This pattern corresponded to where the mice tended to congregate. However, CO2 concentrations did not differ significantly between the 2 cage types. Average CO2 levels in both cage types never exceeded approximately 3000 ppm. Intracage NH3 began to rise in the reusable cages on day 4, reached approximately 50 ppm by day 5 and by day 9 was greater than 150 ppm at the cages' rear sampling port while remaining at approximately 70 ppm at the front sampling port. Intracage NH3 levels in the disposable cages remained less than or equal to 3.2 ppm. Intracage temperature and relative humidity were approximately the same in both cage types. We concluded that the disposable ventilated cage performed satisfactorily under the conditions of the study.
本研究比较了可重复使用和一次性使用的独立通风小鼠笼在笼内二氧化碳和氨气形成方面的情况。将Crl:CD-1(ICR)雌性小鼠置于正压动物饲养架中的一次性或可重复使用的通风笼中。每天测量笼内二氧化碳和氨气浓度,持续9天;前7天监测温度和相对湿度。结果表明,一次性笼子后部和可重复使用笼子前部的二氧化碳水平较高。这种模式与小鼠聚集的位置相对应。然而,两种笼子类型之间的二氧化碳浓度没有显著差异。两种笼子类型的平均二氧化碳水平从未超过约3000 ppm。可重复使用笼子中的笼内氨气在第4天开始上升,到第5天达到约50 ppm,到第9天在笼子后部采样口大于150 ppm,而在前部采样口保持在约70 ppm。一次性笼子中的笼内氨气水平保持在3.2 ppm或以下。两种笼子类型的笼内温度和相对湿度大致相同。我们得出结论,在本研究条件下,一次性通风笼表现令人满意。