蛋白质在固体表面聚集的机制与动力学

Mechanisms and dynamics of protein clustering on a solid surface.

作者信息

Mulheran P A, Pellenc D, Bennett R A, Green R J, Sperrin M

机构信息

Department of Chemical and Process Engineering, University of Strathclyde, James Weir Building, 75 Montrose Street, Glasgow G1 1XJ, United Kingdom.

出版信息

Phys Rev Lett. 2008 Feb 15;100(6):068102. doi: 10.1103/PhysRevLett.100.068102. Epub 2008 Feb 12.

DOI:10.1103/PhysRevLett.100.068102

PMID:18352521

Abstract

A methodology for discovering the mechanisms and dynamics of protein clustering on solid surfaces is presented. In situ atomic force microscopy images are quantitatively compared to Monte Carlo simulations using cluster statistics to differentiate various models. We study lysozyme adsorption on mica as a model system and find that all surface-supported clusters are mobile, not just the monomers, with diffusion constant inversely related to cluster size. The surface monomer diffusion constant is measured to be D(1) approximately 9 x 10(-16) cm(2) s(-1), such a low value being difficult to measure using other techniques.

摘要

本文提出了一种用于发现蛋白质在固体表面聚集的机制和动力学的方法。利用团簇统计将原位原子力显微镜图像与蒙特卡罗模拟进行定量比较，以区分各种模型。我们研究了溶菌酶在云母上的吸附作为一个模型系统，发现所有表面支撑的团簇都是可移动的，不仅仅是单体，其扩散常数与团簇大小成反比。表面单体扩散常数测量为D(1)约为9×10^(-16) cm^2 s^(-1)，如此低的值很难用其他技术测量。