Kimura Masanobu, Tanikawa Tsutomu, Suzuki Michio, Koizumi Nobuo, Kamiyama Tsuneo, Imaoka Koichi, Yamada Akio
Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan.
Microbiol Immunol. 2008 Jan;52(1):9-15. doi: 10.1111/j.1348-0421.2008.00005.x.
Streptobacillus moniliformis is an etiological agent of rat-bite fever and Haverhill fever in human infection. As the currently available methods for identifying the causative bacteria are not satisfactory, we attempted to establish them by PCR using newly designed primers for the 16S rRNA gene of S. moniliformis. We then determined the prevalence of Streptobacillus spp. in two species of feral rats that inhabit an urban region in Japan, because information on the prevalence of the bacteria in feral rats is obscure. The use of PCR with newly designed primers showed that an extremely high proportion of R. norvegicus harbored the bacteria (61/66, 92%), whereas the prevalence was only 58% in R. rattus (30/52). The nucleotide sequence analysis of the 16S rRNA gene of Streptobacillus spp. isolated from oral swabs of feral rats showed at least two different types of bacteria among isolates from R. norvegicus and R. rattus.
念珠状链杆菌是人类感染鼠咬热和哈弗希尔热的病原体。由于目前用于鉴定致病细菌的方法并不令人满意，我们尝试通过PCR，使用新设计的针对念珠状链杆菌16S rRNA基因的引物来进行鉴定。然后，我们测定了日本一个城市地区两种野生鼠类中链杆菌属的流行情况，因为关于野生鼠类中该细菌流行情况的信息尚不明确。使用新设计引物的PCR结果显示，极高比例的褐家鼠携带该细菌（61/66，92%），而黑家鼠中的流行率仅为58%（30/52）。对从野生鼠口腔拭子中分离出的链杆菌属16S rRNA基因进行核苷酸序列分析表明，在褐家鼠和黑家鼠的分离株中至少存在两种不同类型的细菌。
