Wiwanitkit Viroj
Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Pediatr Int. 2008 Apr;50(2):213-5. doi: 10.1111/j.1442-200X.2008.02561.x.
Biliary atresia (BA) is the most common cause of neonatal cholestasis. Recently it was reported that osteopontin (OPN) expression by human bile duct epithelial cells in culture was responsive to interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha). This might be an important part in the pathogenesis of BA, but the function of IL-2 and TNF-alpha in OPN expression is not well understood.
A new gene ontology technology was used to predict the molecular function and biological process of the effect of IL-2 and TNF-alpha on OPN.
Using GoFigure server, the molecular function and biological process of the effect of IL-2 and TNF-alpha on OPN is predicted. Compared to IL-2, TNF-alpha and OPN, the IL-2-TNF-alpha-OPN combination has more functions.
The IL-2-TNF-alpha-OPN combination presents many more functions including positive regulation of osteoclast and response to virus. IL-2 and TNF have a synergistic effect on OPN expression.
胆道闭锁（BA）是新生儿胆汁淤积最常见的病因。最近有报道称，培养的人胆管上皮细胞中骨桥蛋白（OPN）的表达对白细胞介素-2（IL-2）和肿瘤坏死因子-α（TNF-α）有反应。这可能是BA发病机制的重要组成部分，但IL-2和TNF-α在OPN表达中的作用尚不清楚。
采用一种新的基因本体技术预测IL-2和TNF-α对OPN作用的分子功能和生物学过程。
利用GoFigure服务器，预测了IL-2和TNF-α对OPN作用的分子功能和生物学过程。与IL-2、TNF-α和OPN相比，IL-2-TNF-α-OPN组合具有更多功能。
IL-2-TNF-α-OPN组合具有更多功能，包括对破骨细胞的正调控和对病毒的反应。IL-2和TNF对OPN表达有协同作用。
