Lu X, Zhang X H, Wang H, Long X B, You X J, Gao Q X, Cui Y H, Liu Z
Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P R China.
Allergy. 2009 Jan;64(1):104-11. doi: 10.1111/j.1398-9995.2008.01829.x. Epub 2008 Nov 28.
Osteopontin (OPN) is a multifunctional 34-kDa extracellular matrix protein that can influence the inflammatory process. However, the presence of OPN in human sinonasal mucosa and its roles in the inflammatory process of chronic rhinosinusitis (CRS) are not clear. This study investigated the expression of OPN in human sinonasal mucosa, its cytokine-driven expression regulation, and its effect on cytokine production in sinonasal mucosa.
Surgical samples were investigated by means of quantitative reverse transcriptase polymerase chain reaction for evaluation of OPN messenger RNA (mRNA) expression, and the presence and location of OPN protein expression were analyzed using immunohistochemistry. Furthermore, nasal explant culture was used to investigate the mutual regulatory interactions between interferon (IFN)-gamma, interleukin (IL)-4, IL-5, IL-13, IL-1beta, and tumor necrosis factor (TNF)-alpha and OPN in sinonasal mucosa.
Osteopontin expression was significantly upregulated in CRS tissues compared with control tissues. There was a further significant increase of OPN expression in patients with nasal polyps (NPs) and asthma. Immunohistochemistry revealed positive staining of OPN in epithelial cells, submucosal glands, infiltrating cells, and extracellular matrix. Osteopontin mRNA was induced by IFN-gamma, IL-1beta, and TNF-alpha, but inhibited by IL-4 and IL-13. On the contrary, OPN induced IFN-gamma, IL-4, IL-5, IL-13, IL-1beta, and TNF-alpha production in sinonasal mucosa.
The expression of OPN is upregulated in CRS. The mutual regulatory interactions between OPN and inflammatory cytokines suggest that OPN may play an important role in the pathogenesis of CRS.
骨桥蛋白（OPN）是一种多功能的34 kDa细胞外基质蛋白，可影响炎症过程。然而，OPN在人鼻窦黏膜中的存在及其在慢性鼻窦炎（CRS）炎症过程中的作用尚不清楚。本研究调查了OPN在人鼻窦黏膜中的表达、其细胞因子驱动的表达调控及其对鼻窦黏膜中细胞因子产生的影响。
通过定量逆转录聚合酶链反应研究手术样本，以评估OPN信使核糖核酸（mRNA）的表达，并使用免疫组织化学分析OPN蛋白表达的存在和位置。此外，采用鼻外植体培养来研究干扰素（IFN）-γ、白细胞介素（IL）-4、IL-5、IL-13、IL-1β和肿瘤坏死因子（TNF）-α与鼻窦黏膜中OPN之间的相互调节相互作用。
与对照组织相比，CRS组织中骨桥蛋白表达显著上调。鼻息肉（NP）患者和哮喘患者中OPN表达进一步显著增加。免疫组织化学显示上皮细胞、黏膜下腺、浸润细胞和细胞外基质中OPN呈阳性染色。OPN mRNA由IFN-γ、IL-1β和TNF-α诱导，但受IL-4和IL-13抑制。相反，OPN诱导鼻窦黏膜中IFN-γ、IL-4、IL-5、IL-13、IL-1β和TNF-α的产生。
CRS中OPN的表达上调。OPN与炎性细胞因子之间的相互调节相互作用表明，OPN可能在CRS的发病机制中起重要作用。
