[乳腺癌中淋巴转移与血清血管内皮生长因子C及环氧合酶2表达的关系]

[The relationship between lymphatic metastasis and serum vascular endothelial growth factor C and cyclooxygenase 2 expression in breast cancer].

作者信息

Li En-Xiao, Shi Fan, Wu Yin-Ying, Wu Yuan, Guo Jun-Jun, Dong Dan-Feng

机构信息

Cancer Center, First Affiliated Hospital of the School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2008 Jan 8;88(2):88-91.

PMID:18353210

Abstract

OBJECTIVE

To investigate the relationship among the serum vascular endothelial growth factor C (sVEGF-C), expression of cyclooxygenase 2 (COX-2), and lymph vessel density (LMVD), and to discuss their role in tumor progression and lymphatic metastasis.

METHODS

sVEGF-C level was detected by ELISA in 68 pre-operation breast cancer patients, 35 breast benign disease patients, and 12 healthy women. Immunohistochemical method was used to detect the expression of COX-2 and lymphatic vessel endothelial hyaluronidase receptor (LYVE)-1 in the breast cancer tissues and benign disease tissues obtained during operation.

RESULTS

(1) The sVEGF-C level of the pre-operation breast cancer group was (49 +/- 8) pg/ml, significantly higher than those of the begin tumor group [(8 +/- 4) pg/ml, P = 0.045] and the healthy women [(5 +/- 3) pg/ml, P = 0.003]. (2) The COX-2 overexpression positive rate in the breast cancer tissues was 44.1%, significantly higher than that in the begin disease tissues (11.4%, P = 0.002). Higher than that in the begin disease tissue; the LMVD overexpression in the breast cancer tissues was mainly located in the para-carcinoma tissue (8.7 +/- 4.7), significantly higher than that in the begin disease tissues (1.8 +/- 1.7, P = 0.002). (3) The COX-2 overexpression rate of the patients with a high sVEGF-C level was significantly higher than that of the patients with a low sVEGF-C level (P = 0.024). The LMVD level in the breast cancer of the sVEGF-C high level group was (8.7 +/- 3.9), significantly higher than that of the sVEGF-C normal group (5.6 +/- 3.3, P = 0.039). (4) The LMVD in the COX-2 overexpression breast cancer group was 9.5 +/- 3.7, significantly higher than that in the COX-2 negative group (6.0 +/- 3.8, P = 0.012). (5) The sVEGF-C level, COX-2, and LMVD expression in the breast cancer patients with lymphatic metastasis were significantly higher than those in the patients without lymphatic metastasis (P = 0.025, 0.022, and 0.002 respectively).

CONCLUSION

VEGF-C, COX-2, and LMVD play important roles in the lymphatic metastasis of breast cancer. They may be important prognostic factors in evaluating breast cancer lymphatic metastasis.

摘要

目的

探讨血清血管内皮生长因子C（sVEGF-C）、环氧合酶2（COX-2）表达与淋巴管密度（LMVD）之间的关系，并探讨它们在肿瘤进展和淋巴转移中的作用。

方法

采用酶联免疫吸附测定法（ELISA）检测68例乳腺癌术前患者、35例乳腺良性疾病患者及12例健康女性的sVEGF-C水平。采用免疫组织化学方法检测手术中获取的乳腺癌组织及良性疾病组织中COX-2和淋巴管内皮透明质酸受体（LYVE）-1的表达。

结果

（1）乳腺癌术前组sVEGF-C水平为（49±8）pg/ml，显著高于乳腺良性肿瘤组[（8±4）pg/ml，P = 0.045]和健康女性组[（5±3）pg/ml，P = 0.003]。（2）乳腺癌组织中COX-2过表达阳性率为44.1%，显著高于乳腺良性疾病组织（11.4%，P = 0.002）。乳腺癌组织中LMVD过表达主要位于癌旁组织（8.7±4.7），显著高于乳腺良性疾病组织（1.8±1.7，P = 0.002）。（3）sVEGF-C水平高的患者COX-2过表达率显著高于sVEGF-C水平低的患者（P = 0.024）。sVEGF-C高水平组乳腺癌组织中的LMVD水平为（8.7±3.9），显著高于sVEGF-C正常组（5.6±3.3，P = 0.039）。（4）COX-2过表达的乳腺癌组LMVD为9.5±3.7，显著高于COX-2阴性组（6.0±3.8，P = 0.012）。（5）发生淋巴转移的乳腺癌患者的sVEGF-C水平、COX-2及LMVD表达均显著高于未发生淋巴转移的患者（分别为P = 0.025、0.022和0.002）。