Baca Bilgi, Aytac Erman, Apaydin Bedii Berat, Onur Ender, Kilic Ilknur Erenler, Erdamar Sibel, Uslu Ezel, Uzun Hafize, Koksal Selcuk
Department of Surgery, Istanbul University, Cerrahpasa Medical School, Istanbul, Turkey.
Am J Surg. 2008 Sep;196(3):418-24. doi: 10.1016/j.amjsurg.2007.10.005. Epub 2008 Mar 26.
The aim of this study was to evaluate the effects of preoperative mechanical bowel preparation (MBP) on colonic ischemia/reperfusion (I/R) injury.
Seventy adult male Sprague-Dawley rats were divided randomly into 7 equal groups of 10 rats each. Groups were assigned as follows: group I = sham surgery; group II = I/R of left colon (control group); group III = intravenous heparin and metronidazole followed by I/R of the left colon; groups IV through VII = before I/R of the left colon, heparin and metronidazole and MBP were performed with sodium chloride (NaCl), Na phosphate, polyethylene glycol, and mannitol, respectively. Histopathologic and biochemical parameters were evaluated.
According to the histopathologic changes, the groups least affected by I/R injury were groups V and VII. Catalase activity was significantly higher in groups V and VII, and copper-zinc superoxide dismutase activity was significantly higher in group VII compared with the control group (P <.002).
MBP with sodium phosphate and mannitol appears to be more protective against I/R injury.
本研究旨在评估术前机械性肠道准备（MBP）对结肠缺血/再灌注（I/R）损伤的影响。
70只成年雄性Sprague-Dawley大鼠随机分为7组，每组10只。分组如下：I组 = 假手术；II组 = 左半结肠I/R（对照组）；III组 = 静脉注射肝素和甲硝唑后行左半结肠I/R；IV组至VII组 = 在左半结肠I/R前，分别用氯化钠（NaCl）、磷酸钠、聚乙二醇和甘露醇进行肝素、甲硝唑及MBP处理。评估组织病理学和生化参数。
根据组织病理学变化，受I/R损伤影响最小的组是V组和VII组。与对照组相比，V组和VII组的过氧化氢酶活性显著更高，VII组的铜锌超氧化物歧化酶活性显著更高（P <.002）。
磷酸钠和甘露醇进行的MBP似乎对I/R损伤更具保护作用。
