促红细胞生成素对大鼠扭转/去扭转损伤的保护作用。
Protective effect of erythropoietin on torsion/detorsion injury in rat model.
作者信息
Bakan Vedat, Ciralik Harun, Tolun Fatma Inanaç, Atli Yalçin, Mil Ayhan, Oztürk Senol
机构信息
Faculty of Medicine, Department of Pediatric Surgery, Sutcu Imam University, Kahramanmaras 46100, Turkey.
出版信息
J Pediatr Surg. 2009 Oct;44(10):1988-94. doi: 10.1016/j.jpedsurg.2009.02.071.
PURPOSE
The aim of the study is to investigate the effects of erythropoietin on torsion/detorsion injury in rats.
METHODS
Forty rats were divided randomly into 5 groups: group I (sham, S), sham operation; group II (torsion/detorsion 1, T/D(1)), 3 hours ischemia and 1 hour reperfusion; group III (torsion/detorsion 2, T/D(2)), 3 hours ischemia and 48 hours reperfusion; group IV (erythropoietin 1, EPO(1)), 3 hours ischemia, 1 hour reperfusion, and a single dose of EPO; and group V (erythropoietin 2, EPO(2)), 3 hours ischemia, 48 hours reperfusion, and 2 doses of EPO. Malondialdehyde (MDA) and nitric oxide (NO) levels and activities of superoxide dismutase and catalase were measured. Tissue damage to ovarian tissue was scored by histologic examination. Data were compared among groups with parametric tests.
RESULTS
The MDA levels in the S and EPO groups were significantly lower than the T/D groups (P < .001). Catalase and superoxide dismutase activities, and NO levels in the S and EPO groups were significantly higher than in the T/D groups (P < .05). Ovarian tissue damage in the S and EPO groups was significantly less than in the T/D groups (P < .05). Levels of all biochemical markers and ovarian tissue damage scores were similar among the S, EPO(1), and EPO(2) groups (P > .05).
CONCLUSION
Erythropoietin attenuates ischemia-reperfusion injury when given during the acute phase of ovarian torsion-detorsion in a rat model.
目的
本研究旨在探讨促红细胞生成素对大鼠扭转/复位损伤的影响。
方法
40只大鼠随机分为5组：I组（假手术组，S），行假手术；II组（扭转/复位1组，T/D(1)），缺血3小时，再灌注1小时；III组（扭转/复位2组，T/D(2)），缺血3小时，再灌注48小时；IV组（促红细胞生成素1组，EPO(1)），缺血3小时，再灌注1小时，并给予单次剂量促红细胞生成素；V组（促红细胞生成素2组，EPO(2)），缺血3小时，再灌注48小时，并给予2次剂量促红细胞生成素。测定丙二醛（MDA）和一氧化氮（NO）水平以及超氧化物歧化酶和过氧化氢酶的活性。通过组织学检查对卵巢组织损伤进行评分。采用参数检验对各组数据进行比较。
结果
S组和EPO组的MDA水平显著低于T/D组（P <.001）。S组和EPO组的过氧化氢酶和超氧化物歧化酶活性以及NO水平显著高于T/D组（P <.05）。S组和EPO组的卵巢组织损伤明显小于T/D组（P <.05）。S组、EPO(1)组和EPO(2)组的所有生化标志物水平和卵巢组织损伤评分相似（P >.05）。
结论
在大鼠卵巢扭转-复位急性期给予促红细胞生成素可减轻缺血-再灌注损伤。
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