奶牛血清成分分析：凝血血液储存时间和温度的影响

Serum constituents analyses in dairy cows: effects of duration and temperature of the storage of clotted blood.

作者信息

Ehsani A, Afshari A, Bahadori H, Mohri M, Seifi H A

机构信息

Center of Excellence in Ruminant Abortion and Neonatal Mortality, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

出版信息

Res Vet Sci. 2008 Dec;85(3):473-5. doi: 10.1016/j.rvsc.2008.02.004. Epub 2008 Mar 18.

DOI:10.1016/j.rvsc.2008.02.004

PMID:18353408

Abstract

We studied the effects of storage time and temperature of clotted whole blood on the amounts of 17 analytes in bovine blood serum. Serum separated after blood was allowed to stand for 2, 4, 6, 12 and 24h at room temperature or on ice. Results obtained for phosphorous, magnesium, urea, cholesterol, beta-hydroxybutyrate (BHBA), triglyceride, albumin, total protein and gamma-glutamyletransferase (GGT) were not influenced by storage at room temperature or on ice for as long as 24h. Duration of the clotted whole blood storage had a significant effect on calcium, glucose concentrations, creatine kinase (CK) and aspartate aminotransferase (AST) activities and temperature had a significant effect on glucose, non-esterified fatty acids, CK and bilirubin concentrations.

摘要

我们研究了凝血全血的储存时间和温度对牛血清中17种分析物含量的影响。血液在室温或冰上静置2、4、6、12和24小时后分离血清。对于磷、镁、尿素、胆固醇、β-羟基丁酸（BHBA）、甘油三酯、白蛋白、总蛋白和γ-谷氨酰转移酶（GGT），在室温或冰上储存长达24小时，其结果不受影响。凝血全血的储存时间对钙、葡萄糖浓度、肌酸激酶（CK）和天冬氨酸转氨酶（AST）活性有显著影响，而温度对葡萄糖、非酯化脂肪酸、CK和胆红素浓度有显著影响。