França C N, Mendes C C, Ferreira C E S
Pós Graduação em Ciências da Saúde, Universidade Santo Amaro, São Paulo, SP, Brasil.
Setor de Lípides, Aterosclerose e Biologia Vascular, Disciplina de Cardiologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
Braz J Med Biol Res. 2018 Jan 11;51(3):e6955. doi: 10.1590/1414-431X20176955.
The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing of samples immediately after collection.
样本的稳定性对于获取包括血脂谱在内的多种分析物的可靠浓度至关重要。因此,本研究的目的是分析在不同储存和温度条件下的血脂谱。这是一项针对809名男女患者的前瞻性研究。在室温下采集后1小时内、冷藏(8°C)2 - 3小时后以及室温下放置4 - 5小时后,分别测量总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇和非高密度脂蛋白。处理时间和储存条件并未影响所测量的分析物。由于采集后样本立即离心和冷冻存在困难,这些发现对于多中心研究具有重要意义。