Ogo A, Haji M, Ohashi M, Nawata H
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Mol Cell Endocrinol. 1991 Sep;80(1-3):83-9. doi: 10.1016/0303-7207(91)90145-i.
We studied the contents of cortisol (F) and dehydroepiandrosterone (DHEA) and the expression of mRNA of cytochrome P-450 for side-chain cleavage (P-450scc), 17 alpha-hydroxylase (P-450c17), 21 alpha-hydroxylase (P-450c21) and 11 beta-hydroxylase (P-450c11) in adrenocortical adenomas from three patients with Cushing's syndrome. The F content was significantly higher in adrenocortical adenomas than in normal adrenal glands, while the DHEA level was similar to that in normal adrenal glands. The adrenal adenomas showed a markedly higher level of P-450c17 mRNA, and a slightly but not significantly increased level of P-450c21 mRNA, compared with normal adrenal glands. The expression of P-450scc and P-450c11 mRNA in the adenomas was similar to that in normal adrenal glands. These results suggest that the overproduction of cortisol in adrenocortical adenomas associated with Cushing's syndrome results from an increased expression of P-450c17 and P-450c21 mRNA.
我们研究了3例库欣综合征患者肾上腺皮质腺瘤中皮质醇（F）和脱氢表雄酮（DHEA）的含量，以及细胞色素P - 450侧链裂解酶（P - 450scc）、17α - 羟化酶（P - 450c17）、21α - 羟化酶（P - 450c21）和11β - 羟化酶（P - 450c11）的mRNA表达。肾上腺皮质腺瘤中的F含量显著高于正常肾上腺，而DHEA水平与正常肾上腺相似。与正常肾上腺相比，肾上腺腺瘤中P - 450c17 mRNA水平显著升高，P - 450c21 mRNA水平略有升高但无统计学意义。腺瘤中P - 450scc和P - 450c11 mRNA的表达与正常肾上腺相似。这些结果表明，与库欣综合征相关的肾上腺皮质腺瘤中皮质醇的过量产生是由于P - 450c17和P - 450c21 mRNA表达增加所致。
