Jungwirth Britta, Emer Denise, Brune Iris, Hansmeier Nicole, Pühler Alfred, Eikmanns Bernhard J, Tauch Andreas
Centrum für Biotechnologie, Institut für Genomforschung und Systembiologie, Universität Bielefeld, Bielefeld, Germany.
FEMS Microbiol Lett. 2008 Apr;281(2):190-7. doi: 10.1111/j.1574-6968.2008.01098.x. Epub 2008 Mar 18.
The transcriptional regulators RamA, RamB and GlxR were detected to bind to the promoter region of the resuscitation promoting factor 2 (rpf2) gene involved in growth and culturability of Corynebacterium glutamicum. DNA-binding sites were identified by bioinformatic analysis and verified by electrophoretic mobility shift assays with purified hexahistidyl-tagged proteins. Carbon source-dependent deregulation of rpf2 expression was demonstrated in vivo in ramA and ramB mutants and in a C. glutamicum strain overexpressing glxR. The deduced network of regulatory interactions provided insights into the complex regulation pattern of rpf2 expression in C. glutamicum.
已检测到转录调节因子RamA、RamB和GlxR与参与谷氨酸棒杆菌生长和可培养性的复苏促进因子2（rpf2）基因的启动子区域结合。通过生物信息学分析鉴定DNA结合位点，并使用纯化的六组氨酸标签蛋白通过电泳迁移率变动分析进行验证。在ramA和ramB突变体以及过表达glxR的谷氨酸棒杆菌菌株中，体内证实了rpf2表达的碳源依赖性去调节。推导的调控相互作用网络为谷氨酸棒杆菌中rpf2表达的复杂调控模式提供了见解。
