Mamoudou A, Delespaux V, Chepnda V, Hachimou Z, Andrikaye J P, Zoli A, Geerts S
Université de Dschang, Faculté d'Agronomie et des Sciences Agricoles, BP 96, Dschang, Cameroon.
Acta Trop. 2008 May;106(2):115-8. doi: 10.1016/j.actatropica.2008.02.003. Epub 2008 Feb 16.
From May to November 2005, a study was carried out to assess the occurrence of trypanocidal drug resistance (DR) in trypanosomes of naturally infected cattle of the Adamaoua region of Cameroon. Two distinct Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) procedures were used together with an Allele specific-PCR (AS-PCR) and the standardized single-dose mouse test. Using the mouse test, 3 of the 13 Trypanosoma brucei isolates and all 14 tested Trypanosoma congolense isolates were resistant to ISM. However, only 11 of the 25 T. congolense isolates were diagnosed as resistant to ISM using the MboII-PCR-RFLP. Resistance to DA was identified in 1 of the 13 T. brucei isolates and all 11 T. congolense isolates which were tested with the mouse test. Using the AS-PCR or BclI-PCR-RFLP, 3 of the 13 T. brucei isolates and all 25 T. congolense isolates respectively were found resistant. The data presented in this study prove that DR is widespread in the Adamaoua Department of Cameroon. The problem appears to be more serious in T. congolense than in T. brucei. Appropriate measures need to be taken in order to control bovine trypanosomosis in this area.
2005年5月至11月，开展了一项研究，以评估喀麦隆阿达马瓦地区自然感染牛的锥虫中抗锥虫药物耐药性（DR）的发生情况。两种不同的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法与等位基因特异性PCR（AS-PCR）和标准化单剂量小鼠试验一起使用。使用小鼠试验，13株布氏锥虫分离株中的3株以及所有14株测试的刚果锥虫分离株对异硫胂胺（ISM）耐药。然而，使用MboII-PCR-RFLP方法，25株刚果锥虫分离株中只有11株被诊断为对ISM耐药。在13株布氏锥虫分离株中的1株以及所有11株用小鼠试验测试的刚果锥虫分离株中鉴定出对二脒那嗪（DA）的耐药性。使用AS-PCR或BclI-PCR-RFLP方法，分别在13株布氏锥虫分离株中的3株以及所有25株刚果锥虫分离株中发现耐药。本研究提供的数据证明，耐药性在喀麦隆阿达马瓦省广泛存在。该问题在刚果锥虫中似乎比在布氏锥虫中更严重。需要采取适当措施来控制该地区的牛锥虫病。
