Lancelin Frédérique, Djebrani Kayssa, Tabaouti Khalid, Kraoul Linda, Brovedani Sophie, Paubel Pascal, Piketty Marie-Liesse
Department of Biology, Centre Hospitalier Sainte-Anne, Paris, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 May 1;867(1):15-9. doi: 10.1016/j.jchromb.2008.02.026. Epub 2008 Mar 6.
A high-performance liquid chromatography method with diode array detection (HPLC-DAD) was developed for quantification of aripiprazole and dehydro-aripiprazole, in human plasma. After a simple liquid-liquid extraction, chromatographic separation was carried out on a C18 reversed-phase column, using an ammonium buffer-acetonitrile mobile phase (40:60, v/v). The total run time was only 7 min at a flow-rate of 1.0 ml/min. The precision values were less than 12% and the accuracy values were ranging from 98 to 113% and the lower limit of quantification was 2 ng/ml for both compounds. Calibration curves were linear over a range of 2-1000 ng/ml. The mean trough plasma concentrations in patients treated with aripiprazole were 157 and 29 ng/ml for aripiprazole and dehydro-aripiprazole, respectively.
建立了一种采用二极管阵列检测的高效液相色谱法（HPLC-DAD），用于定量测定人血浆中的阿立哌唑和脱氢阿立哌唑。经过简单的液-液萃取后，在C18反相柱上进行色谱分离，使用铵缓冲液-乙腈流动相（40:60，v/v）。在流速为1.0 ml/min时，总运行时间仅为7分钟。两种化合物的精密度值均小于12%，准确度值在98%至113%之间，定量下限均为2 ng/ml。校准曲线在2-1000 ng/ml范围内呈线性。接受阿立哌唑治疗的患者中，阿立哌唑和脱氢阿立哌唑的平均谷浓度分别为157 ng/ml和29 ng/ml。
