Synowiec Anna, Gomółka Ewa, Zyss Tomasz, Zieba Andrzej, Florek Ewa, Piekoszewski Wojciech
Zakład Chemii Analitycznej, Wydział Chemii, Uniwersytet Jagielloński, Kraków.
Przegl Lek. 2012;69(10):1004-6.
Aripiprazole is a new drug applied in schizophrenia treatment. There are not strict indications for aripiprazole therapeutic drug monitoring. Despite, serum aripiprazole measuring would help control the drug doses effectiveness. The drug monitoring can eliminate overdosing, adverse effects and let control proper drug ingestion. The aim of the paper was to develop a simple method for aripiprazole determination in serum for therapeutic drug monitoring. High performance liquid chromatography with spectrophotometric detection (HPLC-UV) was used. Resolution was performed on LC-8 column; moving phase was solution 0,025M trimethylammonium buffer: acetonitrile (62:38). Isocratic flow was 1,2 ml/min; internal standard (IS) was promazine; monitored wavelength was lambda=214 nm. The validation parameters were: limits of linearity (LOL) 100-800 ng/ml, limit of detection (LOD) 10 ng/ml, limit of quantity (LOQ) 100 ng/ml. Coefficient of variation (CV) describing accuracy and precision didn't cross 10%. The method was useful for therapeutic drug monitoring in serum of patients treated with aripiprazole.
阿立哌唑是一种应用于精神分裂症治疗的新药。阿立哌唑治疗药物监测没有严格的指征。尽管如此，测定血清阿立哌唑水平有助于控制药物剂量的有效性。药物监测可以避免用药过量、不良反应，并能控制药物的合理摄入。本文的目的是开发一种用于治疗药物监测的血清中阿立哌唑测定的简单方法。采用高效液相色谱-分光光度检测法（HPLC-UV）。在LC-8柱上进行分离；流动相为0.025M三甲基铵缓冲液：乙腈（62:38）的溶液。等度流速为1.2 ml/min；内标物（IS）为丙嗪；监测波长为λ=214 nm。验证参数为：线性范围（LOL）100 - 800 ng/ml，检测限（LOD）10 ng/ml，定量限（LOQ）100 ng/ml。描述准确度和精密度的变异系数（CV）未超过10%。该方法可用于阿立哌唑治疗患者血清中的治疗药物监测。
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