Soto-Belloso E R, Archbald L F, Zemjanis R
Am J Vet Res. 1976 Sep;37(9):1103-5.
Monolayers of bovine fetal endometrial cells were established as primary culture cells within 1 to 2 weeks. After the 2nd passage, these cells were inoculated with bovine viral diarrhea virus. Effects of the virus were observed each day with a light microscope. Specific cytopathic effects consisting of degeneration and sloughing of the cells and a well-defined pattern of cytoplasmic vacuolation were observed at 5 days after inoculation.
牛胎儿子宫内膜细胞单层在1至2周内被建立为原代培养细胞。在第2次传代后，用牛病毒性腹泻病毒接种这些细胞。每天用光学显微镜观察病毒的作用。接种后5天观察到由细胞变性和脱落以及明确的细胞质空泡化模式组成的特异性细胞病变效应。
