McNulty M S, Allan G M, McFerran J B
Arch Virol. 1977;54(3):201-9. doi: 10.1007/BF01314786.
The growth characteristics in MDBK cells of a calf rotavirus isolated in Northern Ireland are described. Of a range of cell cultures tested, the virus grew in secondary bovine kidney and MDBK cells, but consistently produced a CPE only in MDBK cells. The CPE consisted of cytoplasmic vacuolation, development of eosinophilic intracytoplasmic inclusions, and degeneration and detachment of cells from the monolayer. The onset of CPE was more rapid and its effects more severe in rolled cultures than stationary cultures. Immunofluorescent staining showed that the rotavirus was antigenically unrelated to avian and mammalian reoviruses. There was no significant difference in virus growth over the pH range 6.5 to 8.4.
本文描述了在北爱尔兰分离出的一种小牛轮状病毒在MDBK细胞中的生长特性。在一系列测试的细胞培养物中，该病毒能在二代牛肾细胞和MDBK细胞中生长，但仅在MDBK细胞中持续产生细胞病变效应（CPE）。CPE表现为细胞质空泡化、嗜酸性胞质内包涵体形成以及细胞从单层上退化和脱落。与静止培养相比，在旋转培养中CPE出现得更快且效应更严重。免疫荧光染色显示该轮状病毒与禽和哺乳动物呼肠孤病毒在抗原性上无关。在pH值6.5至8.4范围内，病毒生长没有显著差异。
