Hula N M, Kosiakova H V, Berdyshev A H
Ukr Biokhim Zh (1999). 2007 Sep-Oct;79(5):153-8.
The aim of the presented experiments was to study the influence of suturated NAE--N-stearoylethanolamine (NSE) on the NO synthesis by NO-synthases in aorta and heart tissues of rats with developmental (12-week) streptozotocin-induced (50 mg/kg of body weight) diabetes. Also we evaluated the state of endothelium-dependent relax reactions of aorta smooth muscles. It was shown that the development of diabetes is accompanied with disbalance of NO-synthesis wich consist in inducible NOS (iNOS) activation and inhibition of constitutive NOS (cNOS) and arginase activities. The aorta smooth muscle endothelium-dependent relax reactions were decreased in diabetic rats. The NSE administration to rats with development streptozotocin-induced diabetes resulted in inhibition of iNOS activity and elevation of cNOS and arginase activities in these tissues. Normalization of NO-synthesis under NSE action was accompanied with restoration of aorta smooth muscle endothelium-dependent relax reactions in diabetic rats.
本实验的目的是研究饱和的NAE-N-硬脂酰乙醇胺（NSE）对链脲佐菌素诱导（50mg/kg体重）的发育性（12周）糖尿病大鼠主动脉和心脏组织中一氧化氮合酶合成一氧化氮的影响。我们还评估了主动脉平滑肌内皮依赖性舒张反应的状态。结果表明，糖尿病的发展伴随着一氧化氮合成的失衡，这表现为诱导型一氧化氮合酶（iNOS）的激活以及组成型一氧化氮合酶（cNOS）和精氨酸酶活性的抑制。糖尿病大鼠主动脉平滑肌内皮依赖性舒张反应降低。给链脲佐菌素诱导糖尿病的大鼠施用NSE导致这些组织中iNOS活性受到抑制，而cNOS和精氨酸酶活性升高。在NSE作用下一氧化氮合成的正常化伴随着糖尿病大鼠主动脉平滑肌内皮依赖性舒张反应的恢复。
