人类mRNA中的上游开放阅读框、重新起始和可变翻译起始位点

uORFs, reinitiation and alternative translation start sites in human mRNAs.

作者信息

Kochetov Alex V, Ahmad Shandar, Ivanisenko Vladimir, Volkova Oxana A, Kolchanov Nikolay A, Sarai Akinori

机构信息

Institute of Cytology and Genetics, Lavrentieva Avenue 10, Novosibirsk 630090, Russia.

出版信息

FEBS Lett. 2008 Apr 16;582(9):1293-7. doi: 10.1016/j.febslet.2008.03.014. Epub 2008 Mar 20.

DOI:10.1016/j.febslet.2008.03.014

PMID:18358843

Abstract

It is known that eukaryotic ribosomes are able to translate small ORFs and reinitiate translation at downstream start codons. However, this mechanism is widely considered to be inefficient and it is not commonly taken into account. We compiled a sample of human mRNAs containing small upstream ORFs overlapping with annotated protein coding sequences. Statistical analysis supported the hypothesis on reinitiation of translation at downstream AUG codons and functional significance of potential alternative ORFs. It may be assumed that some 5'UTR-located upstream ORFs can deliver ribosomes to alternative translation starts, and they should be taken into consideration in the prediction of human mRNA coding potential.

摘要

已知真核生物核糖体能够翻译小开放阅读框并在下游起始密码子处重新起始翻译。然而，这种机制被广泛认为效率低下，并且通常未被考虑在内。我们汇编了一个人类mRNA样本，其中包含与注释的蛋白质编码序列重叠的小上游开放阅读框。统计分析支持了关于在下游AUG密码子处重新起始翻译的假设以及潜在替代开放阅读框的功能意义。可以假设一些位于5'非翻译区的上游开放阅读框能够将核糖体传递到替代翻译起始位点，并且在预测人类mRNA编码潜力时应考虑这些开放阅读框。