N-甲基-D-天冬氨酸受体位点与El小鼠癫痫发作的关联

Association of NMDA receptor sites and seizures of El mice.

作者信息

Oguro K, Ito M, Tsuda H, Mutoh K, Shiraishi H, Shirasaka Y, Mikawa H

机构信息

Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.

出版信息

Epilepsy Res. 1991 Sep;9(3):225-30. doi: 10.1016/0920-1211(91)90056-l.

DOI:10.1016/0920-1211(91)90056-l

PMID:1835927

Abstract

To investigate the possible role of N-methyl-D-aspartate (NMDA) receptors in the seizures of El mice, a genetic animal model of epilepsy, we measured [3H]3-[(+)-2-(carboxypiperazin-4-yl)][1,2-3H]- propyl-1-phosphonic acid (CPP) binding in several brain regions of El and ddY mice. At 22-24 weeks of age, the maximum number of binding sites (Bmax) of [3H]CPP was lower only in the cerebral cortex of both stimulated and unstimulated El mice (El(+) and El(-), respectively) than in that of ddY mice. A reduction in Bmax values of cortical [3H]CPP binding of El mice was detected after the age of 12 weeks. Cortical [3H]CPP binding in El(+) mice decreased further transiently after evoked seizures. No significant change was observed in El(-) mice after postural stimulation. These results suggest that El(+) and El(-) mice share seizure propensity and that activation of NMDA receptors is involved in the seizures of El mice.

摘要

为了研究 N-甲基-D-天冬氨酸（NMDA）受体在 El 小鼠（一种癫痫的遗传动物模型）癫痫发作中的可能作用，我们测量了 El 小鼠和 ddY 小鼠几个脑区中[3H]3-[(+)-2-(羧基哌嗪-4-基)][1,2-3H]-丙基-1-膦酸（CPP）的结合情况。在 22 - 24 周龄时，仅在受刺激和未受刺激的 El 小鼠（分别为 El(+)和 El(-)）的大脑皮质中，[3H]CPP 的最大结合位点数（Bmax）低于 ddY 小鼠。在 12 周龄后检测到 El 小鼠皮质[3H]CPP 结合的 Bmax 值降低。诱发癫痫发作后，El(+)小鼠皮质[3H]CPP 结合进一步短暂下降。姿势刺激后，El(-)小鼠未观察到明显变化。这些结果表明，El(+)和 El(-)小鼠具有癫痫发作倾向，并且 NMDA 受体的激活与 El 小鼠的癫痫发作有关。