Tuzen Mustafa, Melek Esra, Soylak Mustafa
Gaziosmanpasa University, Faculty of Science and Arts, Chemistry Department, 60250 Tokat, Turkey.
J Hazard Mater. 2008 Nov 30;159(2-3):335-41. doi: 10.1016/j.jhazmat.2008.02.021. Epub 2008 Feb 16.
Bacillus thuringiensis israelensis loaded on Dowex optipore V-493 as new adsorbent for the separation-preconcentration of heavy metal ions has been proposed. The analytical conditions for the quantitative recoveries of copper(II), iron(III) and zinc(II) including pH, amounts of adsorbent, sample volume, etc. were investigated. The influences of alkaline and earth alkaline ions were also reported. The recovery values for the analytes are generally higher than 95%. The preconcentration factor was 37. The limit of detections of the analyte ions (k=3, N=21) were 1.14 microgL(-1) for copper, 2.01 microgL(-1) for iron and 0.14 microgL(-1) for zinc. The relative standard deviations of the determinations were found to be lower than 9%. The procedure was validated by analyzing copper, iron and zinc contents in two certified reference materials, NRCC-SLRS-4 Riverine water and NIST SRM 1515 Apple leaves. Agreements between the obtained results and the certified values were achieved. The developed preconcentration method was applied in the flame atomic absorption spectrometric determination of copper, iron and zinc in several samples including a multivitamin-multimineral tablet, dialysis solutions, natural waters and some food samples.
已提出将负载于Dowex optipore V - 493上的苏云金芽孢杆菌以色列亚种作为分离富集重金属离子的新型吸附剂。研究了铜（II）、铁（III）和锌（II）定量回收的分析条件，包括pH值、吸附剂用量、样品体积等。还报道了碱金属和碱土金属离子的影响。分析物的回收率通常高于95%。富集倍数为37。分析物离子（k = 3，N = 21）的检测限分别为：铜1.14 μg/L、铁2.01 μg/L、锌0.14 μg/L。测定的相对标准偏差低于9%。通过分析两种有证标准物质NRCC - SLRS - 4河水和NIST SRM 1515苹果叶中的铜、铁和锌含量对该方法进行了验证。获得的结果与认定值相符。所建立的富集方法应用于火焰原子吸收光谱法测定多种样品中的铜、铁和锌，这些样品包括一种多种维生素 - 多种矿物质片剂、透析液、天然水和一些食品样品。
