Huang Cao, Jin Da-di, Zhang Zhong-min, Qu Dong-bin
Department of Orthopedic and Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Mar;28(3):453-6.
To evaluate the biocompatibility of pectin/poly vinyl alcohol composite (CoPP) hydrogel for use as a prosthetic nucleus pulposus material.
The in vitro cytotoxicity of CoPP hydrogel was tested in NCTC L929 cells, which were divided into normal control group, negative control group [treated with poly (vinyl alcohol) hydrogel, PVA], experimental group (treated with CoPP) and positive control group (0.64% phenol). The optical density of the cells on days 2, 4, and 7 of the corresponding treatments was determined and the relative growth rate calculated. For in vivo biocompatibility evaluation, dehydrated CoPP and PVA hydrogel were respectively implanted into the left and right gluteus of SD rats, and the wound healing and general status were observed. The muscular tissues containing the implants were taken 1, 4, and 12 weeks after the implantation for gross observation and microscopic observation of the inflammatory cell infiltration (ICI) and formation of the fibrous capsulation around the implants.
The L929 cells incubated with PVA and CoPP group both grew well, with relative growth rate over 80% and 75%, respectively. The cytotoxicity of PVA and CoPP was both lower than grade 1. In contrast, the relative growth rate in the positive control group was below 24%, with cytotoxicity over grade 4. In the SD rats, ICI of grade IV occurred in the muscular tissues around the PVA and CoPP implants at 1 week without formation of complete capsule, and at 4 weeks, ICI was lowered to grade 1 with grade 4 capsular reaction. Till week 12, the ICI and capsular reaction were both first grade.
CoPP hydrogel has in vitro grade 0 or 1 cytotoxicity and causes only mild inflammation after implantation in rats, suggesting good biocompatibility of the material.
评估果胶/聚乙烯醇复合（CoPP）水凝胶作为人工髓核材料的生物相容性。
在NCTC L929细胞中测试CoPP水凝胶的体外细胞毒性，将细胞分为正常对照组、阴性对照组[用聚乙烯醇（PVA）水凝胶处理]、实验组（用CoPP处理）和阳性对照组（0.64%苯酚）。测定相应处理第2、4和7天细胞的光密度，并计算相对生长率。为评估体内生物相容性，将脱水的CoPP和PVA水凝胶分别植入SD大鼠的左右臀肌，观察伤口愈合情况和一般状态。在植入后1、4和12周取出含植入物的肌肉组织，进行大体观察以及对植入物周围炎症细胞浸润（ICI）和纤维包膜形成进行显微镜观察。
与PVA和CoPP组共培养的L929细胞均生长良好，相对生长率分别超过80%和75%。PVA和CoPP的细胞毒性均低于1级。相比之下，阳性对照组的相对生长率低于24%，细胞毒性超过4级。在SD大鼠中，PVA和CoPP植入物周围肌肉组织在1周时出现IV级ICI，未形成完整包膜，4周时ICI降至1级，包膜反应为4级。至12周时，ICI和包膜反应均为1级。
CoPP水凝胶具有体外0级或1级细胞毒性，在大鼠体内植入后仅引起轻度炎症，表明该材料具有良好的生物相容性。
