Norwitz E R, Starkey P M, López Bernal A, Turnbull A C
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Headington.
J Endocrinol. 1991 Nov;131(2):327-34. doi: 10.1677/joe.0.1310327.
Human term decidua produces prostaglandins (PGs) which have been implicated in the initiation of human parturition. Using flow cytometry to isolate pure cell populations, we have investigated the cell types responsible for decidual PG production. Cell dispersions were prepared enzymatically from decidua vera isolated from term placentae, and were incubated in Dulbecco's Modified Eagle's Medium containing 0.25% bovine serum albumin at 37 degrees C. PGF2 alpha and PGE2 output were measured by radioimmunoassay of the conditioned medium. Production of PGF2 alpha (fmol/10(6) cells per 3 h) exceeded that of PGE2 at 273 (108-322) versus 97 (38-127) respectively (median (range]. The decidual cell dispersions were then incubated with monoclonal antibodies (anti-CD45 which labels the leukocyte common antigen or anti-human leukocyte antigen class II (HLA-DR) which is specific for macrophages in this tissue) and sorted by flow cytometry. The resultant antibody-positive and -negative cell populations were incubated and PG production was measured. Controls showed that antibody labelling and sorting did not alter PG production. PGF2 alpha and PGE2 output by bone marrow-derived (CD45-positive) cell populations exceeded that of non-bone marrow-derived (CD45-negative) cells. Furthermore, we were able to demonstrate that the HLA-DR-positive macrophage population had the highest PGF2 alpha and PGE2 production rates in human term decidua in vitro.
足月人类蜕膜可产生前列腺素（PGs），其与人类分娩的启动有关。我们利用流式细胞术分离纯细胞群体，研究了负责蜕膜PG产生的细胞类型。通过酶法从足月胎盘分离的真蜕膜制备细胞悬液，并在含0.25%牛血清白蛋白的杜尔贝科改良伊格尔培养基中于37℃孵育。通过对条件培养基进行放射免疫测定来测量前列腺素F2α（PGF2α）和前列腺素E2（PGE2）的产量。PGF2α的产量（每3小时每10^6个细胞产生的fmol数）超过PGE2，分别为273（108 - 322）和97（38 - 127）（中位数（范围））。然后将蜕膜细胞悬液与单克隆抗体（标记白细胞共同抗原的抗CD45或针对该组织中巨噬细胞特异的抗人类白细胞抗原II类（HLA - DR））孵育，并通过流式细胞术进行分选。对所得的抗体阳性和阴性细胞群体进行孵育并测量PG产量。对照显示抗体标记和分选不会改变PG产量。骨髓来源（CD45阳性）细胞群体产生的PGF2α和PGE2超过非骨髓来源（CD45阴性）细胞。此外，我们能够证明在体外人足月蜕膜中，HLA - DR阳性巨噬细胞群体具有最高的PGF2α和PGE2产生率。
