围产期单侧缺氧缺血性脑损伤对成年大鼠纹状体多巴胺摄取位点及D1和D2受体的影响。

Effect of unilateral perinatal hypoxic-ischemic brain injury on striatal dopamine uptake sites and D1 and D2 receptors in adult rats.

作者信息

Kostic V S, Przedborski S, Jackson-Lewis V, Cadet J L, Burke R E

机构信息

Department of Neurology, Neurological Institute, Columbia University, College of Physicians & Surgeons, New York, NY 10032.

出版信息

Neurosci Lett. 1991 Aug 19;129(2):197-200. doi: 10.1016/0304-3940(91)90460-b.

DOI:10.1016/0304-3940(91)90460-b

PMID:1836054

Abstract

The effect of unilateral perinatal hypoxic-ischemic brain injury on striatal dopamine uptake sites and on D1 and D2 receptors was investigated in rat by using in vitro quantitative receptor binding autoradiography, 9-11 weeks after the insult. Saturation experiments revealed a significant 20% decrease in maximal binding capacity (Bmax) for [3H]spiperone-labeled D2 receptors on the side of the lesion in comparison to the non-lesioned contralateral side or to either side of control animals. There was no significant change in [3H]mazindol-labeled dopamine uptake sites or in [3H]SCH 23390-labeled D1 receptor characteristics (Bmax and Kd) on the lesioned side. We conclude that the decrease in D2 receptor binding previously observed in immature animals is persistent, whereas the decrease in D1 binding is not.

摘要

在大鼠围产期单侧缺氧缺血性脑损伤9至11周后，通过体外定量受体结合放射自显影技术，研究其对纹状体多巴胺摄取位点以及D1和D2受体的影响。饱和实验显示，与未损伤的对侧或对照动物的任一侧相比，损伤侧[³H]螺哌隆标记的D2受体的最大结合容量（Bmax）显著降低了20%。损伤侧[³H]吗吲哚标记的多巴胺摄取位点或[³H]SCH 23390标记的D1受体特性（Bmax和Kd）没有显著变化。我们得出结论，先前在未成熟动物中观察到的D2受体结合减少是持续存在的，而D1结合的减少则不然。