[过量氟对大鼠切牙成釉细胞中Cbfalpha1表达的影响]
[The effect of overdose fluoride on the expression of Cbfalpha1 in the ameloblasts of rat incisor].
作者信息
Wang Li-Li, Wang Qiang, Wu Hong-Mei, Wei Xiao-Ling, Gao Na
机构信息
Department of Operative Dentistry, College of Stomatology, Tongji University, Shanghai 200072, China.
出版信息
Shanghai Kou Qiang Yi Xue. 2008 Feb;17(1):84-7.
PURPOSE
To evaluate the effect of overdose fluoride on the expression of Cbfalpha1 in the ameloblasts of rat incisor and its correlation with dental fluorosis at protein level.
METHODS
Twenty Wistar rats were divided randomly into two groups, one was fed with distilled water(control group), the other was fed with distilled water of 100ppm F-(experimental group). The incisors of these rats were extracted for immunohistochemical staining eight weeks later to investigate the expression of Cbfalpha1.The data was analyzed with Axioplan 2 imaging system and SPSS10.0 software package.
RESULTS
Cbfalpha1 was expressed in the nuclear of ameloblasts at secretory stage, and the expression level in the experimental group was significantly higher than that in the control group(P<0.01).
CONCLUSION
Overdose fluoride causes higher expression of Cbfalpha1 in the ameloblasts at secretory stage,which may effect the mineralization and development of enamel in a certain way and leads to the abnormal development of enamel.
目的
在蛋白质水平评估过量氟对大鼠切牙成釉细胞中Cbfalpha1表达的影响及其与氟斑牙的相关性。
方法
将20只Wistar大鼠随机分为两组，一组喂蒸馏水（对照组），另一组喂含100ppm氟的蒸馏水（实验组）。8周后拔除这些大鼠的切牙进行免疫组织化学染色，以研究Cbfalpha1的表达。数据用Axioplan 2成像系统和SPSS10.0软件包进行分析。
结果
Cbfalpha1在分泌期成釉细胞核中表达，实验组的表达水平显著高于对照组（P<0.01）。
结论
过量氟导致分泌期成釉细胞中Cbfalpha1表达升高，这可能在一定程度上影响釉质的矿化和发育，导致釉质发育异常。
Zotero 插件