Patterson George H
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
Curr Protoc Cell Biol. 2008 Mar;Chapter 21:Unit 21.6. doi: 10.1002/0471143030.cb2106s38.
A major advance in the microscopic study of cells and tissues is the introduction of photoactivatable fluorescent proteins, which can specifically mark proteins of interest within a living cell. Fluorescent proteins are now available that allow a pool of molecules to be "turned on" by photoactivation. This unit discusses technical aspects for the general use of photoactivatable fluorescent proteins and introduces some specific applications in the concluding remarks.
细胞和组织微观研究的一项重大进展是光激活荧光蛋白的引入，它能够特异性标记活细胞内感兴趣的蛋白质。现在已有荧光蛋白可使一组分子通过光激活而“开启”。本单元讨论光激活荧光蛋白通用技术方面的内容，并在结语中介绍一些具体应用。
