利用共聚焦延时显微镜成像技术观察小鼠发育过程。
Imaging mouse development with confocal time-lapse microscopy.
作者信息
Nowotschin Sonja, Ferrer-Vaquer Anna, Hadjantonakis Anna-Katerina
机构信息
Developmental Biology Program, Sloan-Kettering Institute, New York, USA.
出版信息
Methods Enzymol. 2010;476:351-77. doi: 10.1016/S0076-6879(10)76020-1.
Abstract
The gene expression, signaling, and cellular dynamics driving mouse embryo development have emerged through embryology and genetic studies. However, since mouse development is a temporally regulated three-dimensional process, any insight needs to be placed in this context of real-time visualization. Live imaging using genetically encoded fluorescent protein reporters is pushing the envelope of our understanding by uncovering unprecedented insights into mouse development and leading to the formulation of quantitative accurate models.
摘要
驱动小鼠胚胎发育的基因表达、信号传导和细胞动力学已通过胚胎学和遗传学研究得以揭示。然而，由于小鼠发育是一个受时间调控的三维过程，任何见解都需要置于实时可视化的背景下。使用基因编码荧光蛋白报告基因的活体成像通过揭示对小鼠发育前所未有的见解并促成定量准确模型的建立，正在拓展我们的理解边界。
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