Lippincott-Schwartz Jennifer, Patterson George H
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Methods Cell Biol. 2008;85:45-61. doi: 10.1016/S0091-679X(08)85003-0.
The discoveries, improvements, and alterations of fluorescent protein (FP) variants are having a profound impact on the ability of investigators to observe and quantify the behavior of proteins within cells and organisms. Among the most promising of FPs are photoactivatable fluorescent proteins (PA-FPs). Invisible at the imaging wavelength until activated by irradiation at a different wavelength, PA-FPs allow the controlled highlighting of distinct molecular populations within the cell. This chapter introduces the different types of PA-FPs and discusses their use for monitoring protein movement, protein turnover, protein interactions, and high-resolution protein localization.
荧光蛋白（FP）变体的发现、改进和改变，对研究人员观察和量化细胞及生物体中蛋白质行为的能力产生了深远影响。最具前景的荧光蛋白之一是光激活荧光蛋白（PA-FP）。在成像波长下不可见，直到被不同波长的辐射激活，PA-FP允许对细胞内不同分子群体进行可控的突出显示。本章介绍了不同类型的PA-FP，并讨论了它们在监测蛋白质运动、蛋白质周转、蛋白质相互作用和高分辨率蛋白质定位方面的应用。
