Ahmad Shukur, Singh Vivek Ajit, Hussein Shamsul Iskandar
1 Department of Orthopaedics (NOCERAL), University Malaya Medical Center, Kuala Lumpur, Malaysia.
J Orthop Surg (Hong Kong). 2017 Sep-Dec;25(3):2309499017727946. doi: 10.1177/2309499017727946.
Meniscal allograft transplantation may be a better alternative for the treatment of irreparable meniscal injury compared to other forms of treatment. However, it remains to be seen whether the use fresh frozen allograft is better than cryopreserved allograft in treating this type of injury. We hypothesized that cryopreserved meniscal allograft would work better in maintaining the original biomechanical properties compared to fresh frozen ones, due to the lower amount of damage it incurs during the storage process. We examined young and healthy human menisci obtained from orthopedic oncology patients who underwent resection surgeries around the knee. The menisci obtained were preserved via cryopreservation and deep-freezing process. Traction tests were carried out on the menisci after 6 weeks of preservation. Twelve pairs ( N = 24) of menisci were divided equally into two groups, cryopreservation and deep frozen. There were six males and six female menisci donors for this study. The age range was between 15 and 35 years old (24.9 ± 8.6 years). Cryopreserved specimens had a higher ultimate tensile strength (UTS; 8.2 ± 1.3 Mpa vs. 13.3 ± 1.7 Mpa: p < 0.05) and elastic modulus (61.7 ± 27.6 Mpa vs. 87.0 ± 44.10 Mpa: p < 0.05) compared to the fresh frozen specimens. There was a significant difference in UTS ( p < 0.05) between the two groups but no significant difference in their elastic modulus ( p > 0.05). The elastic modulus of the preserved meniscus was similar to fresh normal menisci taken from other studies (60-120 Mpa; cryopreserved (87.0 ± 44.1) and fresh frozen (61.7 ± 27.5)). Cryopreserved menisci had a higher elastic modulus and point of rupture (UTS) compared to fresh frozen menisci. Cryopreservation proved to be a significantly better method of preservation, among the two methods of preservation in this study.
与其他治疗方式相比，半月板同种异体移植可能是治疗不可修复半月板损伤的更好选择。然而，在治疗这类损伤时，使用新鲜冷冻同种异体移植物是否优于冷冻保存的同种异体移植物仍有待观察。我们假设，由于冷冻保存的半月板同种异体移植物在储存过程中产生的损伤较少，因此与新鲜冷冻的同种异体移植物相比，它在维持原始生物力学特性方面效果更好。我们检查了从接受膝关节周围切除手术的骨科肿瘤患者身上获取的年轻健康人体半月板。所获得的半月板通过冷冻保存和深度冷冻过程进行保存。在保存6周后对半月板进行拉伸试验。12对（N = 24）半月板被平均分为两组，即冷冻保存组和深度冷冻组。本研究有6名男性和6名女性半月板供体。年龄范围在15至35岁之间（24.9 ± 8.6岁）。与新鲜冷冻标本相比，冷冻保存的标本具有更高的极限拉伸强度（UTS；8.2 ± 1.3 Mpa对13.3 ± 1.7 Mpa：p < 0.05）和弹性模量（61.7 ± 27.6 Mpa对87.0 ± 44.10 Mpa：p < 0.05）。两组之间的UTS存在显著差异（p < 0.05），但它们的弹性模量没有显著差异（p > 0.05）。保存的半月板的弹性模量与其他研究中获取的新鲜正常半月板相似（60 - 120 Mpa；冷冻保存的为（87.0 ± 44.1），新鲜冷冻的为（61.7 ± 27.5））。与新鲜冷冻的半月板相比，冷冻保存的半月板具有更高的弹性模量和破裂点（UTS）。在本研究的两种保存方法中，冷冻保存被证明是一种明显更好的保存方法。
