Militzer K
Arch Dermatol Res (1975). 1976 Aug 27;256(2):151-66. doi: 10.1007/BF00567361.
The epidermis and corium of rat right hind paw were investigated both histologically and histometrically after a subplantar injection of aerosil, formalin, carrageenin or Freund's complete adjuvant. The thickness of the epidermis and corium was determined on 5 rats respectively at 12 different times between 0.5 and 480 h post-treatment. Paw swelling was measured plethysmometrically on living rats. The left paw of untreated rats as well as the saline-injected and the untreated left paw of carrageenin-treated and adjuvant-treated animals respectively served as controls. Results 1. After the injection of the 4 substances, an acute inflammation developed maximally 4 to 24 h post-treatment in the corium. The thickness of the corium increased simultaneously with paw swelling. 2. Epidermal hyperplasia set in after a lag-phase of 12 h following the injection of formalin, carrageenin and adjuvant. 3. After injection of aerosil a subcutaneous oedema developed but no epidermal hyperplasia occured. 4. The formation of granulation tissue and necroses in the corium was seen only in the adjuvant-treated group 4 to 20 days post-treatment. At the same time, epidermal hyperplasia reached an absolute maximum. 5. The disturbance of nutrition and the lack of oxygen in the inflamed corium is possibly responsible for the initiation and persistence of the hyperplasia.
在大鼠右后足跖注射气相二氧化硅、福尔马林、角叉菜胶或弗氏完全佐剂后，对其表皮和真皮进行了组织学和组织计量学研究。在治疗后0.5至480小时之间的12个不同时间点，分别对5只大鼠的表皮和真皮厚度进行了测定。用体积描记法测量活鼠的足肿胀情况。未处理大鼠的左足以及角叉菜胶处理和佐剂处理动物的生理盐水注射左足和未处理左足分别作为对照。结果：1. 注射这4种物质后，真皮在治疗后4至24小时出现最大程度的急性炎症。真皮厚度与足肿胀同时增加。2. 在注射福尔马林、角叉菜胶和佐剂12小时的滞后阶段后开始出现表皮增生。3. 注射气相二氧化硅后出现皮下水肿，但未发生表皮增生。4. 仅在佐剂处理组治疗后4至20天，在真皮中可见肉芽组织形成和坏死。同时，表皮增生达到绝对最大值。5. 炎症真皮中的营养紊乱和缺氧可能是增生起始和持续的原因。
