Yamochi Tadanori, Ohnuma Kei, Hosono Osamu, Tanaka Hirotoshi, Kanai Yoshiyuki, Morimoto Chikao
Department of Clinical Immunology, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Biochem Biophys Res Commun. 2008 May 23;370(1):195-9. doi: 10.1016/j.bbrc.2008.03.075. Epub 2008 Mar 24.
We identified human decapping enzyme 2 (hDCP2) as a binding protein with Ro52, being colocalized in processing bodies (p-bodies). We also showed that the N-terminus and C-terminus of Ro52 bound to hDCP2. Moreover, Ro52 enhanced decapping activity of hDCP2 in a dose-dependent manner. Our data support the novel notion of the association between Ro52 with hDCP2 protein in cytoplasmic p-bodies, playing a role in mRNA metabolism in response to cellular stimulation.
我们鉴定出人类去帽酶2（hDCP2）是一种与Ro52结合的蛋白质，二者共定位于加工小体（p小体）中。我们还表明Ro52的N端和C端与hDCP2结合。此外，Ro52以剂量依赖的方式增强了hDCP2的去帽活性。我们的数据支持了Ro52与细胞质p小体中的hDCP2蛋白之间存在关联这一新概念，其在细胞刺激应答的mRNA代谢中发挥作用。
