Ishino S, Kadota K, Matsubara Y, Agawa H, Matsui N
National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
J Vet Med Sci. 1991 Oct;53(5):877-82. doi: 10.1292/jvms.53.877.
Developing lymphoid tissues of bovine fetuses ranging from 70 to 270 days of fetal age were examined by histological and immunohistochemical procedures. In the peripheral blood, surface membrane immunoglobulin bearing cells (B-lymphocytes) and sheep red blood cell rosette forming cells (T-lymphocytes) had already appeared by 70 days of fetal age. In the lymph nodes intracytoplasmic IgM positive cells appeared at 90 days of fetal age. The cells positive for IgG appeared at 150 days of fetal age and IgA positive cells appeared at 180 days of fetal age. The spleen contained intracytoplasmic immunoglobulin positive cells at almost the same time as those in the lymph nodes. In the ileocecal region, IgM positive cells and IgG positive cells were present at 180 days of fetal age and IgA positive cells were present at 210 days of fetal age. The tonsils contained IgM positive cells and IgG positive cells at 240 days of fetal age. In the thymus, terminal deoxynucleotidyl transferase positive cells appeared at 90 days of fetal age.
采用组织学和免疫组织化学方法，对胎龄70至270天的牛胎儿发育中的淋巴组织进行了检查。在胎龄70天时，外周血中已出现表面膜免疫球蛋白阳性细胞（B淋巴细胞）和绵羊红细胞玫瑰花结形成细胞（T淋巴细胞）。在胎龄90天时，淋巴结内出现胞浆IgM阳性细胞。IgG阳性细胞在胎龄150天时出现，IgA阳性细胞在胎龄180天时出现。脾脏中胞浆免疫球蛋白阳性细胞出现的时间与淋巴结几乎相同。在回盲部区域，胎龄180天时存在IgM阳性细胞和IgG阳性细胞，胎龄210天时存在IgA阳性细胞。扁桃体在胎龄240天时含有IgM阳性细胞和IgG阳性细胞。在胸腺中，末端脱氧核苷酸转移酶阳性细胞在胎龄90天时出现。
