Jeyakumar S M, Vajreswari A, Giridharan N V
Division of Biochemistry, National Institute of Nutrition (ICMR), Jamai-Osmania, Hyderabad 500 604, Andhra Pradesh, India.
Biochem Biophys Res Commun. 2008 May 30;370(2):243-7. doi: 10.1016/j.bbrc.2008.03.073. Epub 2008 Mar 24.
Stearoyl-CoA desaturase 1 (SCD1), an important enzyme involved in monounsaturated fatty acid biosynthesis is a key player in energy homeostasis. Here, we tested the impact of vitamin A on hepatic and adipose tissue SCD1 expression and adiposity per se, using an obese mutant rat strain namely, WNIN/Ob developed at National Center for Laboratory Animal Sciences of National Institute of Nutrition, India. Seven months-old 24 male lean and obese rats of WNIN/Ob strain were divided into two groups; each group was subdivided into two subgroups having 6 lean and 6 obese rats and received diets containing either 2.6mg or 129mg vitamin A/kg diet for two months. Feeding of high (but non-toxic) doses of vitamin A resulted in significant reduction in body weight gain, and retroperitoneal white adipose tissue weight (RPWAT) in obese rats. Further, vitamin A feeding resulted in augmented expression of SCD1 in liver and RPWAT of lean rats, while no such effect was seen in obese rats. Taken together, the present data suggest that vitamin A decreases body weight gain in obese rat model independent of SCD1 gene regulation.
硬脂酰辅酶A去饱和酶1（SCD1）是参与单不饱和脂肪酸生物合成的一种重要酶，在能量平衡中起着关键作用。在此，我们使用印度国家营养研究所国家实验动物科学中心培育的一种肥胖突变大鼠品系WNIN/Ob，测试了维生素A对肝脏和脂肪组织中SCD1表达以及肥胖本身的影响。将24只7月龄的WNIN/Ob品系雄性瘦大鼠和肥胖大鼠分为两组；每组再细分为两个亚组，每组有6只瘦大鼠和6只肥胖大鼠，分别给予每千克饲料含2.6毫克或129毫克维生素A的饲料，持续两个月。给肥胖大鼠喂食高剂量（但无毒）的维生素A导致体重增加和腹膜后白色脂肪组织重量（RPWAT）显著降低。此外，喂食维生素A使瘦大鼠肝脏和RPWAT中SCD1的表达增加，而肥胖大鼠未出现这种效应。综上所述，目前的数据表明，在肥胖大鼠模型中，维生素A可降低体重增加，且与SCD1基因调控无关。
