Yang Min, Xu Zhengping, Zhang Ronghua, Zhang Pianhong, Weng Yan, Shen Yueliang, Zhang Xiaoming
School of Medicine, Zhejiang University, 388 Yuhangtang Road, and The Second Affiliated Hospital of Zhejiang University, Hangzhou 310058, P.R. China.
Asia Pac J Clin Nutr. 2008;17(1):23-9.
The myocardial protective effects of water extracts from Hsian-tsao (Mesona procumbens Hemsl.) on diabetic rats were investigated. Thirty Sprague-Dawley male rats were randomly divided into three groups, "control group" (n=10) with intraperitoneal saline injection, "diabetic group" (n=10) with 60 mg of intraperitoneal streptozotocin injection per kg of body weight and "Hsian-tsao group" (n=10) with intragastric administration of Hsian-tsao extracts every day for 4 weeks after intraperitoneal streptozotocin injection. Body weight and blood sugar concentrations were measured before and after model induction in the three groups. Thrombospondin-1 (TSP-1) expressions in the myocardium were monitored by immunohistochemistry and rt-RT-qPCR analysis. Myocardial ultrastructural changes were also analyzed by us-ing transmission electron microscopy. Our results demonstrated that diabetic myocardial ultrastructural changes included myofibrillar disarrangements, mitochondria disruption, and an increase in nuclear membrane invaginations. These damages were significantly less severe in the Hsian-tsao group compared with the diabetic group. A significant increase of the TSP-1 expression was also observed in the hearts of the diabetic rats (p<0.01), but it was relatively lower in the Hsian-tsao group than in the diabetic group (p<0.01). It suggested that Hsian-tsao treatment in the diabetic rats effectively prevented the pathological alterations in the myocardium and decreased TSP-1 expression.
研究了仙草（Mesona procumbens Hemsl.）水提取物对糖尿病大鼠的心肌保护作用。30只雄性Sprague-Dawley大鼠随机分为三组，“对照组”（n = 10）腹腔注射生理盐水，“糖尿病组”（n = 10）腹腔注射链脲佐菌素，剂量为每千克体重60毫克，“仙草组”（n = 10）腹腔注射链脲佐菌素后每天灌胃仙草提取物，持续4周。在模型诱导前后测量三组大鼠的体重和血糖浓度。通过免疫组织化学和rt-RT-qPCR分析监测心肌中血小板反应蛋白-1（TSP-1）的表达。还使用透射电子显微镜分析心肌超微结构变化。我们的结果表明，糖尿病心肌超微结构变化包括肌原纤维排列紊乱、线粒体破坏和核膜内陷增加。与糖尿病组相比，仙草组的这些损伤明显较轻。糖尿病大鼠心脏中TSP-1表达也显著增加（p<0.01），但仙草组低于糖尿病组（p<0.01）。这表明对糖尿病大鼠进行仙草治疗可有效预防心肌的病理改变并降低TSP-1表达。
