Li Xusheng, Hu Ye, Fu Yongqiang, Ying Yanfeng, Chen Guorong
Medical College, Jinhua College of Profession and Technology, Jinhua 321007, China.
Zhongguo Zhong Yao Za Zhi. 2010 Feb;35(3):356-9.
To investigate the effects of Ginkgo biloba extract (GbE) on the glucose uptake rate and gene expression of glucose transporter 4 (GLUT4) in diaphragm of diabetic rats.
Forty SD male rats were randomly divided into normal control group (n=10) and model group (n=30). Diabetic models were induced by feeding with high-sucrose-high-fat diet and intraperitoneal injecting 25 mg X kg(-1) streptozotocin. 20 successful models were rearranged to two groups: diabetic group and GbE treatment group, 10 rats in each. Then the saline and 8 mg X kg(-1) x d(-1) of GbE were respectively intraperitoneal injected, once a day continuously for 8 weeks. The contents of fasting blood glucose (FBG), fasting insulin (FINS) were detected, respectively. The glucose uptake rate and gene expression of GLUT4 in diaphragm were determinated and the varieties of diaphragm ultrastructure were observed.
Compared with control group, levels of FBG and FINS obviously increased in diabetic rats (P < 0.01), but the glucose uptake rate and expression of GLUT4 mRNA in diaphragm decreased significantly (P < 0.05, P < 0.01). The ultrastructure in diabetic group under electron microscope indicated that diaphragm mitochondrion swelled and degenerated. The above changes were inhibited by GbE.
GbE can improve the glucose metabolism in diabetic rats and reduce the diabetes-induced diaphragm damage. The action mechanism of the drug may be related to promote the mRNA expression of GLUT4 in diaphragm and improve the uptake and metabolism of blood glucose.
探讨银杏叶提取物（GbE）对糖尿病大鼠膈肌葡萄糖摄取率及葡萄糖转运蛋白4（GLUT4）基因表达的影响。
40只SD雄性大鼠随机分为正常对照组（n = 10）和模型组（n = 30）。通过高糖高脂饮食喂养及腹腔注射25 mg·kg⁻¹链脲佐菌素诱导糖尿病模型。将20只造模成功的大鼠重新分为两组：糖尿病组和GbE治疗组，每组10只。然后分别腹腔注射生理盐水和8 mg·kg⁻¹·d⁻¹的GbE，每天1次，连续8周。分别检测空腹血糖（FBG）、空腹胰岛素（FINS）含量。测定膈肌葡萄糖摄取率及GLUT4基因表达，并观察膈肌超微结构变化。
与对照组相比，糖尿病大鼠FBG和FINS水平明显升高（P < 0.01），但膈肌葡萄糖摄取率及GLUT4 mRNA表达显著降低（P < 0.05，P < 0.01）。糖尿病组电镜下超微结构显示膈肌线粒体肿胀、变性。上述变化被GbE抑制。
GbE可改善糖尿病大鼠的糖代谢，减轻糖尿病所致的膈肌损伤。该药物的作用机制可能与促进膈肌GLUT4 mRNA表达、改善血糖摄取和代谢有关。
