Chen H T, Casanova M F, Kleinman J E, Zito M, Goldman D, Linnoila M
Laboratory of Clinical Studies, DICBR, National Institute of Alcohol Abuse and Alcoholism (NIAAA), Bethesda, MD.
Psychiatry Res. 1991 Sep;38(3):293-9. doi: 10.1016/0165-1781(91)90019-l.
High affinity 3H-paroxetine binding was studied in human frontal cortex and hippocampus obtained from normal controls and alcoholics. On the basis of Scatchard analyses, a significant decrease in the maximal number of binding sites (Bmax) was found in the hippocampus of alcoholics (n = 8) as compared with that of controls (n = 10) (mean +/- SD = 63 +/- 35 vs. 114 +/- 70 fmoles/mg protein). There was no significant difference in the dissociation constants (Kd) between the two groups. The presumed effect of chronic alcohol abuse on 3H-paroxetine binding may be region-specific since no significant difference in either Bmax or Kd for 3H-paroxetine binding was found in the frontal cortex between normal controls and alcoholics. No significant correlation of 3H-paroxetine binding with age or postmortem interval was observed. The decrease in 3H-paroxetine binding in the hippocampus of alcoholics is probably indicative of reduced density of serotonergic nerve terminals either as a preexisting condition or as a result of neuronal damage caused by ethanol or the sequelae of alcoholism, such as nutritional deficiencies.
在取自正常对照组和酗酒者的人类额叶皮质和海马体中研究了高亲和力3H-帕罗西汀结合情况。基于Scatchard分析，发现酗酒者（n = 8）海马体中结合位点的最大数量（Bmax）与对照组（n = 10）相比显著降低（均值±标准差= 63±35对114±70飞摩尔/毫克蛋白质）。两组之间的解离常数（Kd）没有显著差异。长期酗酒对3H-帕罗西汀结合的假定影响可能具有区域特异性，因为在正常对照组和酗酒者的额叶皮质中，3H-帕罗西汀结合的Bmax或Kd均未发现显著差异。未观察到3H-帕罗西汀结合与年龄或死后间隔之间存在显著相关性。酗酒者海马体中3H-帕罗西汀结合的减少可能表明血清素能神经末梢密度降低，这可能是一种预先存在的状况，或者是由乙醇或酗酒后遗症（如营养缺乏）导致的神经元损伤的结果。
