使用ADVIA 120对马血细胞计数进行评估，并与阻抗计数器和手工分类计数法进行比较。

Evaluation of equine hemograms using the ADVIA 120 as compared with an impedance counter and manual differential count.

作者信息

Giordano Alessia, Rossi Gabriele, Pieralisi Cristina, Paltrinieri Saverio

机构信息

Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Milan, Italy.

出版信息

Vet Clin Pathol. 2008 Mar;37(1):21-30. doi: 10.1111/j.1939-165X.2008.00012.x.

DOI:10.1111/j.1939-165X.2008.00012.x

PMID:18366541

Abstract

BACKGROUND

The ADVIA 120 is an automated laser cell counter widely used in veterinary medicine. Although specific software for equine samples is available and validated, only a few reports have been published comparing the ADVIA 120 with other methods for equine hemogram evaluation.

OBJECTIVES

The purpose of this study was to compare the hematologic values and reference intervals obtained on the ADVIA 120 with those obtained on an impedance cell counter and manual differential counts in healthy horses.

METHODS

EDTA-anticoagulated blood samples were obtained from 114 clinically healthy horses of various breeds, both sexes, and 2-6 years of age. Samples were stored for up to 12 hours at 4 degrees C and then analyzed on the ADVIA 120 and the Hemat 8. A 100-cell to 200-cell differential leukocyte count was performed by 3 independent observers on May-Grünwald-Giemsa-stained smears. Intra-assay precision of the ADVIA 120 was determined by analyzing 5 replicates each of 10 of the blood samples.

RESULTS

Results from the ADVIA were significantly higher than those from the impedance counter for RBC count, total WBC count, hemoglobin concentration, red cell distribution width, MCH, and MCHC, and significantly lower for HCT and platelet count. Significantly higher neutrophil and basophil counts and significantly lower lymphocyte counts were obtained with the ADVIA 120 compared with manual counts. Based on Passing-Bablok regression analysis, RBC and platelet counts were in good agreement between the 2 analyzers; a constant and proportional bias was present for other values. Coefficients of variation for erythrocyte parameters on the ADVIA were <1%, but were higher for platelet (6%), total WBC (2%), differential WBC (4%-30%), and reticulocyte (75%) counts.

CONCLUSIONS

Results obtained with equine samples on the ADVIA 120 were comparable with those obtained on an impedance counter; reference intervals differed statistically but overlapped. The ADVIA had poor precision for reticulocyte and differential leukocyte counts such that the latter should always be verified on smears.

摘要

背景

ADVIA 120是一种在兽医学中广泛使用的自动激光血细胞计数器。尽管有适用于马样本的特定软件且已得到验证，但仅有少数报告发表，比较了ADVIA 120与其他用于马血液学检查评估的方法。

目的

本研究的目的是比较在健康马匹中，ADVIA 120所获得的血液学值和参考区间与阻抗血细胞计数器及手工分类计数所获得的结果。

方法

从114匹2至6岁、不同品种、性别各异的临床健康马匹采集乙二胺四乙酸（EDTA）抗凝血液样本。样本在4℃下保存长达12小时，然后在ADVIA 120和Hemat 8上进行分析。由3名独立观察者对May-Grünwald-Giemsa染色涂片进行100至200个白细胞的分类计数。通过分析10份血液样本中的每份样本的5个重复来确定ADVIA 120的批内精密度。

结果

ADVIA得出的红细胞计数、白细胞总数、血红蛋白浓度、红细胞分布宽度、平均红细胞血红蛋白含量（MCH）和平均红细胞血红蛋白浓度（MCHC）结果显著高于阻抗计数器得出的结果，而血细胞比容（HCT）和血小板计数则显著低于阻抗计数器得出的结果。与手工计数相比，ADVIA 120得出的中性粒细胞和嗜碱性粒细胞计数显著更高，淋巴细胞计数显著更低。基于Passing-Bablok回归分析，两种分析仪之间的红细胞和血小板计数一致性良好；其他值存在恒定和比例偏差。ADVIA上红细胞参数的变异系数<1%，但血小板（6%）、白细胞总数（2%）、白细胞分类计数（4%-30%）和网织红细胞计数（75%）的变异系数更高。