Bonsembiante Federico, Guglielmini Carlo, Berlanda Michele, Fiocco Pietro, Biancani Barbara, Genovese Carla, Bedin Silvia, Gelain Maria Elena
Department of Animal Medicine, Production and Health, University of Padua, 35020 Legnaro, PD, Italy.
Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, PD, Italy.
Animals (Basel). 2023 Apr 11;13(8):1313. doi: 10.3390/ani13081313.
Hematological analyses are particularly useful in assessing a dolphin's health status. However, the creation of appropriate reference intervals for this species is difficult due to the low number of reference individuals. The implementation of individual reference intervals (iRIs) allows researchers to overcome this limitation and, moreover, also consider the within-individual variability. The aims of this study were (1) to evaluate the biological variations in some hematological measurands, including erythrocytes (RBC), hematocrit (Hct), mean cellular volume and hemoglobin content (MCV and MCHC, respectively), RBC distribution width (RDW), leukocytes (WBC), and platelets (PLT); and (2) to calculate the index of individuality (IoI) and reference change value (RCV), which enable the production of iRIs, in healthy managed bottlenose dolphins. Seven dolphins were included, and the results of six hematological exams were analyzed for each animal. Analytical imprecision (CV), within-dolphin variation (CV), and between-dolphins variations (CV) were calculated, and the IoI and RCV were derived for each measurand. All the hematological measurands had intermediate IoI except WBC, for which Iol was low. The calculated RCV ranged from 10.33% (MCV) to 186.51% (WBC). The results reveal that the majority of hematological measurands have an intermediate level of individuality in dolphins, and thus the application of iRIs is appropriate. The calculated RCV can also be applied to other managed dolphins and could be useful in interpreting serial CBC exams.
血液学分析在评估海豚的健康状况方面特别有用。然而，由于可供参考的个体数量较少，为该物种建立合适的参考区间很困难。实施个体参考区间（iRIs）使研究人员能够克服这一限制，而且还能考虑个体内部的变异性。本研究的目的是：（1）评估一些血液学测量指标的生物学变异，包括红细胞（RBC）、血细胞比容（Hct）、平均细胞体积和血红蛋白含量（分别为MCV和MCHC）、红细胞分布宽度（RDW）、白细胞（WBC）和血小板（PLT）；（2）计算个体性指数（IoI）和参考变化值（RCV），这有助于为健康管理的宽吻海豚生成iRIs。研究纳入了7只海豚，对每只动物的6项血液学检查结果进行了分析。计算了分析不精密度（CV）、海豚个体内部变异（CV）和海豚个体之间的变异（CV），并得出了每个测量指标的IoI和RCV。除白细胞的IoI较低外，所有血液学测量指标的IoI均处于中等水平。计算得出的RCV范围为10.33%（MCV）至186.51%（WBC）。结果表明，大多数血液学测量指标在海豚中具有中等程度的个体性，因此应用iRIs是合适的。计算得出的RCV也可应用于其他管理的海豚，并有助于解释连续的全血细胞计数检查结果。
