文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

PICK1在N-甲基-D-天冬氨酸受体依赖的双向突触可塑性中起重要作用。

An essential role for PICK1 in NMDA receptor-dependent bidirectional synaptic plasticity.

作者信息

Terashima Akira, Pelkey Kenneth A, Rah Jong-Cheol, Suh Young Ho, Roche Katherine W, Collingridge Graham L, McBain Chris J, Isaac John T R

机构信息

Developmental Synaptic Plasticity Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 35 Convent Drive, Bethesda, MD 20892, USA.

出版信息

Neuron. 2008 Mar 27;57(6):872-82. doi: 10.1016/j.neuron.2008.01.028.

DOI:10.1016/j.neuron.2008.01.028
PMID:18367088
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2336895/
Abstract

PICK1 is a calcium-sensing, PDZ domain-containing protein that interacts with GluR2 and GluR3 AMPA receptor (AMPAR) subunits and regulates their trafficking. Although PICK1 has been principally implicated in long-term depression (LTD), PICK1 overexpression in CA1 pyramidal neurons causes a CaMK- and PKC-dependent potentiation of AMPAR-mediated transmission and an increase in synaptic GluR2-lacking AMPARs, mechanisms associated with NMDA receptor (NMDAR)-dependent long-term potentiation (LTP). Here, we directly tested whether PICK1 participates in both hippocampal NMDAR-dependent LTP and LTD. We show that the PICK1 potentiation of AMPAR-mediated transmission is NMDAR dependent and fully occludes LTP. Conversely, blockade of PICK1 PDZ interactions or lack of PICK1 prevents LTP. These observations demonstrate an important role for PICK1 in LTP. In addition, deletion of PICK1 or blockade of PICK1 PDZ binding prevented NMDAR-dependent LTD. Thus, PICK1 plays a critical role in bidirectional NMDAR-dependent long-term synaptic plasticity in the hippocampus.

摘要

PICK1是一种含PDZ结构域的钙敏感受体蛋白，它与AMPA受体（AMPAR）的GluR2和GluR3亚基相互作用并调节其转运。尽管PICK1主要与长时程抑制（LTD）有关，但在CA1锥体神经元中过表达PICK1会导致CaMK和PKC依赖的AMPAR介导的传递增强以及缺乏GluR2的突触性AMPAR增加，这些机制与NMDA受体（NMDAR）依赖的长时程增强（LTP）有关。在这里，我们直接测试了PICK1是否参与海马体中NMDAR依赖的LTP和LTD。我们发现，PICK1对AMPAR介导传递的增强作用依赖于NMDAR，并且完全阻断了LTP。相反，阻断PICK1的PDZ相互作用或缺乏PICK1会阻止LTP。这些观察结果证明了PICK1在LTP中起着重要作用。此外，缺失PICK1或阻断PICK1的PDZ结合会阻止NMDAR依赖的LTD。因此，PICK1在海马体中双向NMDAR依赖的长期突触可塑性中起关键作用。

相似文献

[1]
An essential role for PICK1 in NMDA receptor-dependent bidirectional synaptic plasticity.

Neuron. 2008-3-27

[2]
Calcium binding to PICK1 is essential for the intracellular retention of AMPA receptors underlying long-term depression.

J Neurosci. 2010-12-8

[3]
PDZ protein mediated activity-dependent LTP/LTD developmental switch at rat retinocollicular synapses.

Am J Physiol Cell Physiol. 2010-3-24

[4]
Identification of a small-molecule inhibitor of the PICK1 PDZ domain that inhibits hippocampal LTP and LTD.

Proc Natl Acad Sci U S A. 2009-12-14

[5]
Targeted in vivo mutations of the AMPA receptor subunit GluR2 and its interacting protein PICK1 eliminate cerebellar long-term depression.

Neuron. 2006-3-16

[6]
Distinct trafficking and expression mechanisms underlie LTP and LTD of NMDA receptor-mediated synaptic responses.

Hippocampus. 2010-5

[7]
Role of AMPA receptor trafficking in NMDA receptor-dependent synaptic plasticity in the rat lateral amygdala.

J Neurochem. 2008-7

[8]
Lipid binding regulates synaptic targeting of PICK1, AMPA receptor trafficking, and synaptic plasticity.

J Neurosci. 2006-3-1

[9]
Input- and subunit-specific AMPA receptor trafficking underlying long-term potentiation at hippocampal CA3 synapses.

Eur J Neurosci. 2004-7

[10]
Differential regulation of AMPA receptor trafficking by neurabin-targeted synaptic protein phosphatase-1 in synaptic transmission and long-term depression in hippocampus.

J Neurosci. 2007-4-25

引用本文的文献

[1]
Protein interacting with C-kinase 1 (PICK1) regulates synaptic function and reversal learning in a mouse model for schizophrenia.

Neuropsychopharmacology. 2025-2-22

[2]
Neural Influences on Tumor Progression Within the Central Nervous System.

CNS Neurosci Ther. 2024-10

[3]
MET receptor tyrosine kinase promotes the generation of functional synapses in adult cortical circuits.

Neural Regen Res. 2025-5-1

[4]
Sex-Dependent Differences in the Ischemia/Reperfusion-Induced Expression of AMPA Receptors.

Int J Mol Sci. 2024-2-13

[5]
ICA69 regulates activity-dependent synaptic strengthening and learning and memory.

Front Mol Neurosci. 2023-5-12

[6]
In the fast lane: Receptor trafficking during status epilepticus.

Epilepsia Open. 2023-5

[7]
Heterozygote Dopamine Transporter Knockout Rats Display Enhanced Cocaine Locomotion in Adolescent Females.

Int J Mol Sci. 2022-12-6

[8]
Disrupted Maturation of Prefrontal Layer 5 Neuronal Circuits in an Alzheimer's Mouse Model of Amyloid Deposition.

Neurosci Bull. 2023-6

[9]
Ca Regulates Dimerization of the BAR Domain Protein PICK1 and Consequent Membrane Curvature.

Front Mol Neurosci. 2022-6-2

[10]
Neurons and Astrocytes Elicit Brain Region Specific Transcriptional Responses to Prion Disease in the Murine CA1 and Thalamus.

Front Neurosci. 2022-5-16

本文引用的文献

[1]
PICK1 and phosphorylation of the glutamate receptor 2 (GluR2) AMPA receptor subunit regulates GluR2 recycling after NMDA receptor-induced internalization.

J Neurosci. 2007-12-12

[2]
PICK1-ICA69 heteromeric BAR domain complex regulates synaptic targeting and surface expression of AMPA receptors.

J Neurosci. 2007-11-21

[3]
A GluR1-cGKII interaction regulates AMPA receptor trafficking.

Neuron. 2007-11-21

[4]
Kalirin-7 controls activity-dependent structural and functional plasticity of dendritic spines.

Neuron. 2007-11-21

[5]
Age-dependent requirement of AKAP150-anchored PKA and GluR2-lacking AMPA receptors in LTP.

EMBO J. 2007-11-28

[6]
Developmental expression of Ca2+-permeable AMPA receptors underlies depolarization-induced long-term depression at mossy fiber CA3 pyramid synapses.

J Neurosci. 2007-10-24

[7]
Long-term potentiation in the hippocampal CA1 region does not require insertion and activation of GluR2-lacking AMPA receptors.

J Neurophysiol. 2007-10

[8]
The role of the GluR2 subunit in AMPA receptor function and synaptic plasticity.

Neuron. 2007-6-21

[9]
Conservation of glutamate receptor 2-containing AMPA receptors during long-term potentiation.

J Neurosci. 2007-4-25

[10]
Increased expression of the immediate-early gene arc/arg3.1 reduces AMPA receptor-mediated synaptic transmission.

Neuron. 2006-11-9

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索