瘦素及瘦素受体在牛腺垂体中的定位

Localization of leptin and leptin receptor in the bovine adenohypophysis.

作者信息

Ogasawara Hideki, Ohwada Shyuichi, Nagai Yasuhiro, Taketa Yoshikazu, Matsuzaki Masatoshi, Tanaka Sachi, Watanabe Kouichi, Aso Hisashi, Yamaguchi Takahiro

机构信息

Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555 Sendai, Japan.

出版信息

Domest Anim Endocrinol. 2008 Jul;35(1):8-15. doi: 10.1016/j.domaniend.2008.01.006. Epub 2008 Mar 17.

DOI:10.1016/j.domaniend.2008.01.006

PMID:18367366

Abstract

The present study was carried out to detail the cellular localization of leptin (Lep) and the leptin receptor (LepR) in the bovine adenohypophysis. Lep immunoreactivity (Lep-ir) was found in about 30% of adenohypophysial cells in the gland. Immunochemistry of Lep and specific hormones using serial sections revealed that Lep-ir was present in 60.4% of somatotrophs, 15.9% of gonadotrophs, 6.5% of mammotrophs, 6.5% of thyrotrophs and 2.4% of corticotrophs. Both the common short isoform (OBRa) and the long isoform (OBRb) of LepR mRNA were expressed in the bovine adenohypophysis. LepR immunoreactivity (LepR-ir) was found in only 2.8% of the adenohypophysial cells and over 50% of LepR-ir cells were gonadotrophs, in which most of the cells were distributed in the zona tuberalis. The findings on Lep and LepR in the adenohypophysial cells indicate that Lep may regulate gonadotroph function through autocrine/paracrine pathway in the bovine adenohypophysis.

摘要

本研究旨在详细阐述瘦素（Lep）和瘦素受体（LepR）在牛腺垂体中的细胞定位。在腺体内约30%的腺垂体细胞中发现了瘦素免疫反应性（Lep-ir）。使用连续切片对瘦素和特定激素进行免疫化学分析显示，Lep-ir存在于60.4%的生长激素细胞、15.9%的促性腺激素细胞、6.5%的催乳激素细胞、6.5%的促甲状腺激素细胞和2.4%的促肾上腺皮质激素细胞中。LepR mRNA的常见短异构体（OBRa）和长异构体（OBRb）均在牛腺垂体中表达。仅在2.8%的腺垂体细胞中发现了LepR免疫反应性（LepR-ir），且超过50%的LepR-ir细胞为促性腺激素细胞，其中大多数细胞分布在结节部。腺垂体细胞中关于瘦素和瘦素受体的研究结果表明，在牛腺垂体中，瘦素可能通过自分泌/旁分泌途径调节促性腺激素细胞的功能。