Kong Lu, Tang Meng, Zhang Ting, Wang Dayong, Hu Ke, Lu Weiqi, Wei Chao, Liang Geyu, Pu Yuepu
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Medical School of Southeast University, Nanjing 210009, China.
Int J Mol Sci. 2014 Nov 17;15(11):21253-69. doi: 10.3390/ijms151121253.
Nickel is associated with reproductive toxicity. However, the reproductive toxicity of nickel nanoparticles (Ni NPs) is unclear. Our goal was to determine the association between nickel nanoparticle exposure and reproductive toxicity. According to the one-generation reproductive toxicity standard, rats were exposed to nickel nanoparticles by gavage and we selected indicators including sex hormone levels, sperm motility, histopathology, and reproductive outcome etc. Experimental results showed nickel nanoparticles increased follicle stimulating hormone (FSH) and luteinizing hormone (LH), and lowered etradiol (E2) serum levels at a dose of 15 and 45 mg/kg in female rats. Ovarian lymphocytosis, vascular dilatation and congestion, inflammatory cell infiltration, and increase in apoptotic cells were found in ovary tissues in exposure groups. For male rats, the weights decreased gradually, the ratio of epididymis weight over body weight increased, the motility of rat sperm changed, and the levels of FSH and testosterone (T) diminished. Pathological results showed the shedding of epithelial cells of raw seminiferous tubule, disordered arrangement of cells in the tube, and the appearance of cell apoptosis and death in the exposure group. At the same time, Ni NPs resulted in a change of the reproductive index and the offspring development of rats. Further research is needed to elucidate exposure to human populations and mechanism of actions.
镍与生殖毒性有关。然而，镍纳米颗粒（Ni NPs）的生殖毒性尚不清楚。我们的目标是确定镍纳米颗粒暴露与生殖毒性之间的关联。根据一代生殖毒性标准，通过灌胃法让大鼠暴露于镍纳米颗粒中，我们选择了包括性激素水平、精子活力、组织病理学和生殖结局等指标。实验结果显示，在雌性大鼠中，剂量为15和45mg/kg的镍纳米颗粒会使促卵泡激素（FSH）和黄体生成素（LH）升高，而雌二醇（E2）血清水平降低。在暴露组的卵巢组织中发现了卵巢淋巴细胞增多、血管扩张和充血、炎症细胞浸润以及凋亡细胞增加。对于雄性大鼠，体重逐渐下降，附睾重量与体重之比增加，大鼠精子活力改变，FSH和睾酮（T）水平降低。病理结果显示，暴露组生精小管上皮细胞脱落，管内细胞排列紊乱，出现细胞凋亡和死亡。同时，镍纳米颗粒导致大鼠生殖指数和子代发育发生变化。需要进一步研究以阐明其对人群的暴露情况和作用机制。
