Frerich B, Zückmantel K, Winter K, Müller-Dürwald S, Hemprich A
Department of Oral and Maxillofacial Surgery, Facial Plastic Surgery, University of Leipzig, Leipzig, Germany.
Int J Oral Maxillofac Surg. 2008 May;37(5):459-66. doi: 10.1016/j.ijom.2008.01.014. Epub 2008 Mar 25.
In an effort to engineer capillary-like networks in vitro, different cultivation methods were compared. Five small-diameter tube-like constructs ('vessel equivalents') were fabricated from porcine gelatin scaffolds and seeded with human adipose tissue stromal cells and umbilical vein endothelial cells. After initial growth, the vessel equivalents were divided. One segment was exposed to pulsatile perfusion and the other was kept in rotating culture. Specimens harvested at the start of the experiments and after 16 days of rotation or perfusion were compared histomorphometrically with respect to capillary-like network formation in the vessel wall. Most capillary-like structures were found in the luminal portion of perfused vessel equivalents. Maturation of these capillary-like structures ascertained by recruitment of alpha-actin-positive cells also reached the highest degree in the luminal portion of perfused specimens. Perfused specimens showed significantly less apoptosis. Pulsatile perfusion promotes the development and maturation of a capillary-like network in this in-vitro approach.
为了在体外构建类毛细血管网络，对不同的培养方法进行了比较。用猪明胶支架制作了五个小直径管状构建物（“血管等效物”），并接种了人脂肪组织基质细胞和脐静脉内皮细胞。初始生长后，将血管等效物分开。一部分暴露于搏动灌注，另一部分保持在旋转培养中。将实验开始时以及旋转或灌注16天后收获的标本在组织形态计量学上比较血管壁中类毛细血管网络的形成情况。在灌注的血管等效物的管腔部分发现了大多数类毛细血管结构。通过募集α-肌动蛋白阳性细胞确定的这些类毛细血管结构的成熟在灌注标本的管腔部分也达到了最高程度。灌注标本显示出明显较少的细胞凋亡。在这种体外方法中，搏动灌注促进了类毛细血管网络的发育和成熟。
