从尿路感染中分离的 的分子检测及药敏评价。
Molecular Detection of and Antibiotic Susceptibility Evaluation of Isolated from Urinary Tract Infections.
机构信息
Department of Medical Laboratory Techniques, Faculty of Medical and Health Techniques, University of Alkafeel, Najaf, Iraq.
2Department of Anesthesia Techniques, Faculty of Medical and Health Techniques, University of Alkafeel, Najaf, Iraq.
出版信息
Arch Razi Inst. 2022 Apr 30;77(2):573-578. doi: 10.22092/ARI.2022.357153.1985. eCollection 2022 Apr.
The present study aimed to isolate and diagnose () from clinical specimens of patients infected with urinary tract infections and evaluate the bacteria's resistance to antimicrobial agents. Additionally, it attempted to study the existence of the () gene. This study took place in Najaf province, Iraq, from December 2020 to April 2021 and included 40 clinical specimens taken from urine. In order to make an initial diagnosis of isolates, microscopic evaluation was used in conjunction with culture and biochemical features. The automatic final diagnostic provided by the VITEK-2 compact system (bioMérieux, France) was utilized, which had a significant advantage. The results showed that 27 (67.5%) isolates gave positive results for , 2 (5%) isolates for , 6 (15%) isolates for , and 5 (12.5%) for . Antibiotic sensitivity test was conducted by disk diffusion methods, in which the isolates showed high resistance to ceftriaxone, as well as erythromycin, and they showed sensitivity to vancomycin, gentamicin, amikacin, and ciprofloxacin. The findings led to a concluding remark that all of the isolates were gene positive.
本研究旨在从尿路感染患者的临床标本中分离和诊断 ()，并评估细菌对抗菌药物的耐药性。此外，还试图研究 () 基因的存在。本研究于 2020 年 12 月至 2021 年 4 月在伊拉克纳杰夫省进行，共采集了 40 份来自尿液的临床标本。为了初步诊断 () 分离株，我们结合培养和生化特征进行了显微镜评估。我们使用了 VITEK-2 紧凑型系统（法国生物梅里埃）提供的自动最终诊断，这具有显著的优势。结果显示，27 株（67.5%）分离株对 () 呈阳性，2 株（5%）对 () 呈阳性，6 株（15%）对 () 呈阳性，5 株（12.5%）对 () 呈阳性。我们通过纸片扩散法进行了抗生素敏感性试验，结果显示，这些分离株对头孢曲松、红霉素具有高度耐药性，对万古霉素、庆大霉素、阿米卡星和环丙沙星敏感。研究结果表明，所有的 () 分离株均为 () 基因阳性。
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